Rabbit anti-Human, Mouse MEKKK 4 Polyclonal Antibody | anti-MEKKK 4 antibody

MEKKK 4 Antibody

Cat. Num. AAA9504897

• Gene Names: MAP4K4; HGK; NIK; MEKKK4; FLH21957; HEL-S-31
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: MEKKK 4; Polyclonal Antibody; MEKKK 4 Antibody; HPK/GCK-like kinase HGK; M4K4; MAP4K4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; Nck interacting kinase; hepatocyte progenitor kinase-like/germinal center kinase-like kinase; mitogen-activated protein kinase kinase kinase kinase 4; anti-MEKKK 4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: MEKKK 4 Antibody detects endogenous levels of total MEKKK 4 protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1,273

• Applicable Applications for anti-MEKKK 4 antibody: Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IF: 1:100~1:500; ELISA: 1:5000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human MEKKK 4.
• Immunogen Range: 406-455
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells, using MEKKK 4 Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from HUVEC and COLO cells, using MEKKK 4 Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 336020358
• NCBI GeneID: 9448
• NCBI Accession #: NP_001229488.1
• NCBI GenBank Nucleotide #: NM_001242559.1
• UniProt Accession #: O95819; O75172; Q9NST7; E7ESS2
• Molecular Weight: 142 kDa
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 4 isoform 4
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 4
• NCBI Official Symbol: MAP4K4
• NCBI Official Synonym Symbols: HGK; NIK; MEKKK4; FLH21957; HEL-S-31
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 4
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 4
• UniProt Gene Name: MAP4K4
• UniProt Synonym Gene Names: HGK; KIAA0687; NIK; MEK kinase kinase 4; MEKKK 4

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.