Western Blot (WB)
(Western Blot analysis of ARHGAP15 expression in transfected 293T cell line by ARHGAP15 polyclonal antibody. Lane 1: ARHGAP15 transfected lysate (52.25kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human ARHGAP15 Polyclonal Antibody | anti-ARHGAP15 antibody
ARHGAP15 (Rho GTPase-activating Protein 15, ArhGAP15, Rho-type GTPase-activating Protein 15, BM-024, BM-030, BM-046)
Gene Names
ARHGAP15; BM046
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
ARHGAP15; Polyclonal Antibody; ARHGAP15 (Rho GTPase-activating Protein 15; ArhGAP15; Rho-type GTPase-activating Protein 15; BM-024; BM-030; BM-046); Anti -ARHGAP15 (Rho GTPase-activating Protein 15; anti-ARHGAP15 antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ARHGAP15.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MQKSTNSDTSVETLNSTRQGTGAVQMRIKNANSHHDRLSQSKSMILTDVGKVTEPISRHRRNHSQHILKDVIPPLEQLMVEKEGYLQKAKIADGGKKLRKNWSTSWIVLSSRRIEFYKESKQQALSNMKTGHKPESVDLCGAHIEWAKEKSSRKNVFQITTVSGNEFLLQSDIDFIILDWFHAIKNAIDRLPKDSSCPSRNLELFKIQRSSSTELLSHYDSDIKEQKPEHRKSLMFRLHHSASDTSDKNRVKSRLKKFITRRPSLKTLQEKGLIKDQIFGSHLHKVCERENSTVPWFVKQCIEAVEKRGLDVDGIYRVSGNLATIQKLRFIVNQEEKLNLDDSQWEDIHVVTGALKMFFRELPEPLFPYSFFEQFVEAIKKQDNNTRIEAVKSLVQKLPPPNRDTMKVLFGHLTKIVAKASKNLMSTQSLGIVFGPTLLRAENETGNMAIHMVYQNQIAELMLSEYSKIFGSEED
Applicable Applications for anti-ARHGAP15 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Full length human ARHGAP15, aa1-475 (AAH38976.2).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of ARHGAP15 expression in transfected 293T cell line by ARHGAP15 polyclonal antibody. Lane 1: ARHGAP15 transfected lysate (52.25kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of ARHGAP15 expression in transfected 293T cell line by ARHGAP15 polyclonal antibody. Lane 1: ARHGAP15 transfected lysate (52.25kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-ARHGAP15 antibody
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction.
Product Categories/Family for anti-ARHGAP15 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
54,544 Da
NCBI Official Full Name
ARHGAP15
NCBI Official Synonym Full Names
Rho GTPase activating protein 15
NCBI Official Symbol
ARHGAP15
NCBI Official Synonym Symbols
BM046
NCBI Protein Information
rho GTPase-activating protein 15; rho-type GTPase-activating protein 15
UniProt Protein Name
Rho GTPase-activating protein 15
Protein Family
UniProt Gene Name
ARHGAP15
UniProt Entry Name
RHG15_HUMAN
NCBI Description
RHO GTPases (see ARHA; MIM 165390) regulate diverse biologic processes, and their activity is regulated by RHO GTPase-activating proteins (GAPs), such as ARHGAP15 (Seoh et al., 2003 [PubMed 12650940]).[supplied by OMIM, Mar 2008]
Uniprot Description
ARHGAP15: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction.
Protein type: GAPs, Rac/Rho; GAPs
Chromosomal Location of Human Ortholog: 2q22.2-q22.3
Cellular Component: membrane; cytosol
Biological Process: regulation of cell shape; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction
Research Articles on ARHGAP15
Similar Products
Product Notes
The ARHGAP15 arhgap15 (Catalog #AAA6004870) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ARHGAP15 (Rho GTPase-activating Protein 15, ArhGAP15, Rho-type GTPase-activating Protein 15, BM-024, BM-030, BM-046) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGAP15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the ARHGAP15 arhgap15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MQKSTNSDTS VETLNSTRQG TGAVQMRIKN ANSHHDRLSQ SKSMILTDVG KVTEPISRHR RNHSQHILKD VIPPLEQLMV EKEGYLQKAK IADGGKKLRK NWSTSWIVLS SRRIEFYKES KQQALSNMKT GHKPESVDLC GAHIEWAKEK SSRKNVFQIT TVSGNEFLLQ SDIDFIILDW FHAIKNAIDR LPKDSSCPSR NLELFKIQRS SSTELLSHYD SDIKEQKPEH RKSLMFRLHH SASDTSDKNR VKSRLKKFIT RRPSLKTLQE KGLIKDQIFG SHLHKVCERE NSTVPWFVKQ CIEAVEKRGL DVDGIYRVSG NLATIQKLRF IVNQEEKLNL DDSQWEDIHV VTGALKMFFR ELPEPLFPYS FFEQFVEAIK KQDNNTRIEA VKSLVQKLPP PNRDTMKVLF GHLTKIVAKA SKNLMSTQSL GIVFGPTLLR AENETGNMAI HMVYQNQIAE LMLSEYSKIF GSEED. It is sometimes possible for the material contained within the vial of "ARHGAP15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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