MEK7 Polyclonal Antibody | anti-MEK7 antibody

Anti-MEK7 Antibody

Cat. Num. AAA178114

• Gene Names: MAP2K7; MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: MEK7; Polyclonal Antibody; Anti-MEK7 Antibody; Dual specificity mitogen-activated protein kinase kinase 7; c Jun N terminal kinase kinase 2; c-Jun N-terminal kinase kinase 2; Dual specificity mitogen activated protein kinase kinase 7; JNK activating kinase 2; JNK kinase 2; JNK-activating kinase 2; JNKK 2; Jnkk-2; Jnkk2; MAP kinase kinase 7; MAP2K 7; MAP2K7; MAPK/ERK kinase 7; MAPKK 7; MAPKK-7; MAPKK7; MEK 7; Mitogen activated protein kinase kinase 7; MKK 7; MKK-7; MKK7; MP2K7_HUMAN; PRKMK 7; PRKMK-7; PRKMK7; Protein kinase mitogen activated kinase 7; Sek 2; Sek-2; Sek2; SKK4 antibody; mitogen-activated protein kinase kinase 7; anti-MEK7 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 435

• Applicable Applications for anti-MEK7 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MEK7 (2-40aa AASSLEQKLSRLEAKLKQENREARRRIDLNLDISPQRPR), identical to the related mouse and rat sequences.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- MEK7 Picoband antibody, MBS178114, Western blottingAll lanes: Anti MEK7 (MBS178114) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: NIH3T3 Whole Cell Lysate at 40ugPredicted bind size: 47KDObserved bind size: 47KD )

Immunohistochemistry (IHC)

(Anti- MEK7 Picoband antibody, MBS178114,IHC(P)IHC(P): Mouse Lung Tissue )

Immunohistochemistry (IHC)

(Anti- MEK7 Picoband antibody, MBS178114,IHC(P)IHC(P): Rat Lung Tissue )

Immunohistochemistry (IHC)

(Anti- MEK7 Picoband antibody, MBS178114,IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Related Product Information for anti-MEK7 antibody

Description: Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 7(MAP2K7) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: MAP2K7(Mitogen-activated protein kinase kinase 7), also known as MAP kinase kinase 7, MAPKK7, JNKK2, PRKMK7 or MKK7, is an enzyme that in humans is encoded by the MAP2K7 gene. This protein is a member of the mitogen-activated protein kinase kinase family. The MKK7 protein exists as six different isoforms with three possible N-termini (alpha, beta, and gamma isoforms) and two possible C-termini (1 and 2 isoforms). Schramek et al. (2011) showed that the doxorubicin-mediated DNA damage response in human A549 lung carcinoma cells caused rapid phosphorylation and upregulation of p53 (TP53). MKK7 knockdown reduced p53 phosphorylation, delayed p53 upregulation, and interfered with cell cycle arrest at G2/M. MKK7 was activated in primary lung tumors, and tumors with a p53 mutation showed even higher MKK7 phosphorylation. Schramek et al. (2011) concluded that MKK7 exerts its tumor suppressive function through p53.

References

1. Lu, X., Nemoto, S., Lin, A. Identification of c-jun NH(2)-terminal protein kinase (JNK)-activating kinase 2 as an activator of JNK but not p38. J. Biol. Chem. 272: 24751-24754, 1997. 2. Schramek, D., Kotsinas, A., Meixner, A., Wada, T., Elling, U., Pospisilik, J. A., Neely, G. G., Zwick, R.-H., Sigl, V., Forni, G., Serrano, M., Gorgoulis, V. G., Penninger, J. M. The stress kinase MKK7 couples oncogenic stress to p53 stability and tumor suppression. Nature Genet. 43: 212-219, 2011. 3. Wu, Z., Wu, J., Jacinto, E., Karin, M. Molecular cloning and characterization of human JNKK2, a novel jun NH(2)-terminal kinase-specific kinase. Molec. Cell. Biol. 17: 7407-7416, 1997.

NCBI and Uniprot Product Information

• NCBI GI #: 662033893
• NCBI GeneID: 5609
• NCBI Accession #: NP_001284484.1
• NCBI GenBank Nucleotide #: NM_001297555.1
• UniProt Accession #: O14733; O14648; O14816; O60452; O60453; Q1PG43; Q8IY10; B2R9S5; D6W659
• Molecular Weight: 48,182 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 7 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 7
• NCBI Official Symbol: MAP2K7
• NCBI Official Synonym Symbols: MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 7
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 7
• UniProt Gene Name: MAP2K7
• UniProt Synonym Gene Names: JNKK2; MEK7; MKK7; PRKMK7; SKK4; MAP kinase kinase 7; MAPKK 7; MEK 7; SAPK kinase 4; SAPKK-4; SAPKK4; JNK kinase 2; JNKK 2
• UniProt Entry Name: MP2K7_HUMAN

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

MKK7: a dual-specificity protein kinase of the STE7 family. Activates JNK1 and -2 by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by proinflammatory cytokines and environmental stress. Three alternatively spliced isoforms have been reported.

Protein type: Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; Kinase, protein; EC 2.7.12.2; STE group; STE7 family

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: ATP binding; enzyme binding; JUN kinase kinase activity; magnesium ion binding; MAP kinase kinase activity; mitogen-activated protein kinase kinase kinase binding; protein binding; protein C-terminus binding; protein kinase binding; protein phosphatase binding; protein-tyrosine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: activation of JNK activity; apoptosis; JNK cascade; peptidyl-tyrosine phosphorylation; positive regulation of neuron apoptosis; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; response to heat; response to osmotic stress; response to UV; signal transduction; stress-activated MAPK cascade