Western Blot (WB)
((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat ACO1/Aconitase 1 Polyclonal Antibody | anti-ACO1 antibody
Goat anti-ACO1/Aconitase 1 Antibody
Western Blot: Approx 100kDa band observed in Human Liver lysates (calculated MW of 98.4kDa according to NP_002188.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]
Uniprot Description
IREB1: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Belongs to the aconitase/IPM isomerase family.
Protein type: RNA-binding; Endoplasmic reticulum; Lyase; Carbohydrate Metabolism - citrate (TCA) cycle; EC 4.2.1.3; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Translation
Chromosomal Location of Human Ortholog: 9p21.1
Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; Golgi apparatus; mitochondrion
Molecular Function: 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; iron-responsive element binding; metal ion binding; protein binding; RNA binding
Biological Process: cellular iron ion homeostasis; citrate metabolic process; intestinal absorption; post-embryonic development; regulation of translation; response to iron(II) ion; tricarboxylic acid cycle
Research Articles on ACO1
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Product Notes
The ACO1 aco1 (Catalog #AAA421648) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ACO1/Aconitase 1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ACO1/Aconitase 1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 100kDa band observed in Human Liver lysates (calculated MW of 98.4kDa according to NP_002188.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the ACO1 aco1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QVDFNRRADS LQKNQ. It is sometimes possible for the material contained within the vial of "ACO1/Aconitase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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