Western Blot (WB)
(Western blot analysis of CD61 (pY773) expression in HUVEC (A), Raw264.7 (B) whole cell lysates.)
CD61 Polyclonal Antibody | anti-ITGB3 antibody
Anti-CD61 (pY773) Phospho Antibody
Western Blot (WB)
(Western blot analysis of CD61 (pY773) expression in HUVEC (A), Raw264.7 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of CD61 (pY773) expression in HUVEC (A), Raw264.7 (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of CD61 (pY773) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of CD61 (pY773) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]
Uniprot Description
ITGB3: integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface.
Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 17q21.32
Cellular Component: filopodium membrane; platelet alpha granule membrane; focal adhesion; cell surface; integral to plasma membrane; microvillus membrane; melanosome; plasma membrane; nucleus; integrin complex; receptor complex
Molecular Function: identical protein binding; protein binding; protease binding; vascular endothelial growth factor receptor 2 binding; extracellular matrix binding; fibronectin binding; platelet-derived growth factor receptor binding; protein disulfide isomerase activity; receptor activity; cell adhesion molecule binding
Biological Process: negative regulation of lipid transport; axon guidance; extracellular matrix organization and biogenesis; entry of virus into host cell; protein folding; wound healing; cell-matrix adhesion; smooth muscle cell migration; negative chemotaxis; mesodermal cell differentiation; positive regulation of vascular endothelial growth factor receptor signaling pathway; platelet degranulation; cell-substrate junction assembly; cell growth; cell adhesion; integrin-mediated signaling pathway; platelet activation; cell migration; regulation of bone resorption; negative regulation of lipoprotein metabolic process; angiogenesis involved in wound healing; cell-substrate adhesion; heterotypic cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of low-density lipoprotein receptor biosynthetic process; activation of protein kinase activity; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; blood coagulation; tube development; vascular endothelial growth factor receptor signaling pathway; leukocyte migration
Disease: Glanzmann Thrombasthenia; Myocardial Infarction, Susceptibility To; Bleeding Disorder, Platelet-type, 16
Research Articles on ITGB3
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Product Notes
The ITGB3 itgb3 (Catalog #AAA8222887) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD61 (pY773) Phospho Antibody reacts with Human, Mouse, Rat, Bovine, Dog, Monkey, Porcine, Rabbit, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's CD61 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IHC (1/100 - 1/200). Researchers should empirically determine the suitability of the ITGB3 itgb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD61, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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