Rabbit MEK5 Polyclonal Antibody | anti-MEK5 antibody

MEK5 Rabbit Polyclonal

Cat. Num. AAA7601350

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: MEK5; Polyclonal Antibody; MEK5 Rabbit Polyclonal; anti-MEK5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 438

• Applicable Applications for anti-MEK5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Mitogen-activated protein kinase kinase 5
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human heart using MBS7601350 (MAP2K5 antibody) at dilution of 1:50)

Immunofluorescence (IF)

(Immunofluorescent analysis of U2OS cells, using MAP2K5 antibody MBS7601350 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red) .)

Western Blot (WB)

(HeLa cells were subjected to SDS PAGE followed by western blot with MBS7601350 (MAP2K5 antibody) at dilution of 1:500)

NCBI and Uniprot Product Information

• NCBI GI #: 1255720
• Molecular Weight: 46,131 Da
• NCBI Official Full Name: MEK5
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 5
• UniProt Gene Name: MAP2K5
• UniProt Synonym Gene Names: MEK5; MKK5; PRKMK5; MAP kinase kinase 5; MAPKK 5; MEK 5
• UniProt Entry Name: MP2K5_HUMAN

Uniprot Description

MEK5: a dual specificity protein kinase of the STE7 family. Activates ERK5 and is itself phosphorylated and activated by MEKK3, as well as by atypical protein kinase C isoforms. Involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced isoforms have been described.

Protein type: Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); EC 2.7.12.2; STE group; STE7 family

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: cytoplasm; cytosol; nucleus; spindle

Molecular Function: ATP binding; metal ion binding; protein binding; protein kinase activity; protein-tyrosine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; heart development; inhibition of NF-kappaB transcription factor; negative regulation of caspase activity; negative regulation of heterotypic cell-cell adhesion; negative regulation of interleukin-8 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; peptidyl-tyrosine phosphorylation; positive regulation of cell growth; positive regulation of epithelial cell proliferation; positive regulation of protein metabolic process; positive regulation of transcription from RNA polymerase II promoter; signal transduction