Mouse Galectin-3/LGALS3 ELISA Kit | LGALS3 elisa kit

Mouse Galectin-3/LGALS3 PicoKine ELISA Kit

Cat. Num. AAA176522

• Gene Names: Lgals3; GBP; L-34; gal3; Mac-2
• Reactivity: Mouse
• Synonyms: Galectin-3/LGALS3; Mouse Galectin-3/LGALS3 PicoKine ELISA Kit; Galectin-3; lectin; galactoside-binding; soluble; 3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Gal-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; GALIG; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; LGALS2Mac-2 antigen; MAC2; Macrophage galactose-specific lectin; LGALS3 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse Galectin-3
• Sequence Length: 264

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse Galectin-3/LGALS3 PicoKine ELISA Kit standard curve )

Related Product Information for LGALS3 elisa kit

Principle of the assay: mouse Galectin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Galectin-3 has been precoated onto 96-well plates. Standards (E.coli,A2-I264) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Galectin-3 amount of sample captured in plate.
Background: Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21-q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 81876134
• NCBI GeneID: 16854
• UniProt Accession #: Q8C253; P16110
• Molecular Weight: 27,415 Da
• NCBI Official Full Name: NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630104P19 product:lectin, galactose binding, soluble 3, full insert sequence (NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630
• NCBI Official Synonym Full Names: lectin, galactose binding, soluble 3
• NCBI Official Symbol: Lgals3
• NCBI Official Synonym Symbols: GBP; L-34; gal3; Mac-2
• NCBI Protein Information: galectin-3; gal-3; CBP 35; lectin L-29; 35 kDa lectin; mac-2 antigen; igE-binding protein; laminin-binding protein; galactose-specific lectin 3; L-34 galactoside-binding lectin; carbohydrate-binding protein 35
• UniProt Protein Name: Galectin
• UniProt Gene Name: Lgals3
• UniProt Entry Name: Q8C253_MOUSE

Uniprot Description

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.

Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Cell surface

Cellular Component: spliceosome; extracellular matrix; proteinaceous extracellular matrix; extracellular space; cell surface; membrane; cytoplasm; mitochondrial inner membrane; extracellular region; immunological synapse; nucleus; external side of plasma membrane

Molecular Function: monosaccharide binding; protein binding; advanced glycation end-product receptor activity; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity; disaccharide binding

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; immune system process; positive regulation of serotonin secretion; RNA splicing; negative regulation of endocytosis; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; signal transduction; macrophage chemotaxis; monocyte chemotaxis; positive regulation of angiogenesis; positive chemotaxis; positive regulation of cell proliferation; eosinophil chemotaxis; innate immune response; mRNA processing; cell differentiation; skeletal development; negative regulation of apoptosis