Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (MED9 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MED9 Antibody detected the MED9 protein (arrow).)

Rabbit MED9 Polyclonal Antibody | anti-MED9 antibody

MED9 Antibody (C-term)

Gene Names
MED9; MED25
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MED9; Polyclonal Antibody; MED9 Antibody (C-term); Mediator of RNA polymerase II transcription subunit 9; Mediator complex subunit 9; MED25; anti-MED9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MED9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-136 amino acids from the C-terminal region of human MED9.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
107-136
Sequence Length
146
Applicable Applications for anti-MED9 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(MED9 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MED9 Antibody detected the MED9 protein (arrow).)

Western Blot (WB) (MED9 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MED9 Antibody detected the MED9 protein (arrow).)
Related Product Information for anti-MED9 antibody
The multiprotein Mediator complex is a coactivator
required for activation of RNA polymerase II transcription by DNA
bound transcription factors. The protein encoded by this gene is
thought to be a subunit of the Mediator complex. This gene is
located within the Smith-Magenis syndrome region on chromosome 17.
References
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Bi, W., et al. Genome Res. 12(5):713-728(2002)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16403
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 9
NCBI Official Synonym Full Names
mediator complex subunit 9
NCBI Official Symbol
MED9
NCBI Official Synonym Symbols
MED25
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 9
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 9
UniProt Gene Name
MED9
UniProt Synonym Gene Names
MED25
UniProt Entry Name
MED9_HUMAN

NCBI Description

The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

MED9: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 9 family.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: Srb-mediator complex

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter

Research Articles on MED9

Similar Products

Product Notes

The MED9 med9 (Catalog #AAA9202493) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 107-136. The MED9 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's MED9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the MED9 med9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.