Rabbit anti-Human, Mouse MED9 Polyclonal Antibody | anti-MED9 antibody

MED9, CT (MED9, MED25, Mediator of RNA polymerase II transcription subunit 9, Mediator complex subunit 9) (APC)

Cat. Num. AAA6320079

• Gene Names: MED9; MED25
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MED9; Polyclonal Antibody; CT (MED9; MED25; Mediator of RNA polymerase II transcription subunit 9; Mediator complex subunit 9) (APC); Mediator complex subunit 9; anti-MED9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MED9 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MED9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-136 amino acids from the C-terminal region of human MED9.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MED9 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MED9 Antibody detected the MED9 protein (arrow).)

Related Product Information for anti-MED9 antibody

The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17.

Product Categories/Family for anti-MED9 antibody

Antibodies; Transcription Factors

References

Sato, S., et al. Mol. Cell 14(5):685-691(2004). Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004). Bi, W., et al. Genome Res. 12(5):713-728(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 8922273
• NCBI GeneID: 55090
• NCBI Accession #: NP_060489.1
• NCBI GenBank Nucleotide #: NM_018019.2
• UniProt Accession #: Q9NWA0; Q8VCS6
• Molecular Weight: 16,403 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 9
• NCBI Official Synonym Full Names: mediator complex subunit 9
• NCBI Official Symbol: MED9
• NCBI Official Synonym Symbols: MED25
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 9; mediator subunit 25; mediator of RNA polymerase II transcription, subunit 9 homolog
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 9
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED9
• UniProt Synonym Gene Names: MED25
• UniProt Entry Name: MED9_HUMAN

NCBI Description

The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

MED9: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 9 family.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: Srb-mediator complex

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter