Western Blot (WB)
(MED6 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MED6 antibody detected the MED6 protein (arrow).)
Rabbit anti-Human MED6 Polyclonal Antibody | anti-MED6 antibody
MED6, CT (MED6, ARC33, Mediator of RNA polymerase II transcription subunit 6, Activator-recruited cofactor 33kD component, Mediator complex subunit 6, Renal carcinoma antigen NY-REN-28) (Azide free) (HRP)
Gene Names
MED6; ARC33; NY-REN-28
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MED6; Polyclonal Antibody; CT (MED6; ARC33; Mediator of RNA polymerase II transcription subunit 6; Activator-recruited cofactor 33kD component; Mediator complex subunit 6; Renal carcinoma antigen NY-REN-28) (Azide free) (HRP); Renal carcinoma antigen NY-REN-28; anti-MED6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-MED6 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
MED6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-246 amino acids from the C-terminal region of human MED6.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(MED6 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MED6 antibody detected the MED6 protein (arrow).)
Western Blot (WB)
(MED6 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MED6 antibody detected the MED6 protein (arrow).)
Related Product Information for anti-MED6 antibody
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Product Categories/Family for anti-MED6 antibody
References
Pavri, R., et al. Mol. Cell 18(1):83-96(2005). Sato, S., et al. Mol. Cell 14(5):685-691(2004). Yang, F., et al. Proc. Natl. Acad. Sci. U.S.A. 101(8):2339-2344(2004). Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004). Sato, S., et al. J. Biol. Chem. 278(50):49671-49674(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28425
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 6 isoform 2
NCBI Official Synonym Full Names
mediator complex subunit 6
NCBI Official Symbol
MED6
NCBI Official Synonym Symbols
ARC33; NY-REN-28
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 6
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 6
Protein Family
UniProt Gene Name
MED6
UniProt Synonym Gene Names
ARC33; ARC33; hMed6
UniProt Entry Name
MED6_HUMAN
Uniprot Description
MED6: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 6 family.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 14q24.2
Cellular Component: nucleoplasm; membrane; Srb-mediator complex; nucleus
Molecular Function: protein binding; transcription coactivator activity; transcription factor binding
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; gene expression
Similar Products
Product Notes
The MED6 med6 (Catalog #AAA6320038) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MED6, CT (MED6, ARC33, Mediator of RNA polymerase II transcription subunit 6, Activator-recruited cofactor 33kD component, Mediator complex subunit 6, Renal carcinoma antigen NY-REN-28) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MED6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MED6 med6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
