Rabbit anti-Human CLIC5 Polyclonal Antibody | anti-CLIC5 antibody

CLIC5 (Chloride Intracellular Channel Protein 5) (MaxLight 490)

Cat. Num. AAA6469456

• Gene Names: CLIC5; MST130; DFNB102; DFNB103; MSTP130
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CLIC5; Polyclonal Antibody; CLIC5 (Chloride Intracellular Channel Protein 5) (MaxLight 490); anti-CLIC5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CLIC5.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-CLIC5 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Human recombinant CLIC5 protein.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CLIC5 Antibody in K562 cell line lysates (35ug/lane). CLIC5(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with CLIC5 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-CLIC5 antibody

Antibodies; Ion Channel

References

1. Berryman, M., J. Biol. Chem. 279(33), 34794-34801 (2004). 2. Suzuki, T., Epilepsy Res. 50(3), 265-275 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 82617632
• NCBI GeneID: 53405
• NCBI Accession #: NP_058625.2
• NCBI GenBank Nucleotide #: NM_016929.4
• UniProt Accession #: Q9NZA1; Q5T4Z0; Q8NBY3; Q96JT5; Q9BWZ0; B3KUF1
• Molecular Weight: 22,830 Da
• NCBI Official Full Name: chloride intracellular channel protein 5 isoform b
• NCBI Official Synonym Full Names: chloride intracellular channel 5
• NCBI Official Symbol: CLIC5
• NCBI Official Synonym Symbols: MST130; DFNB102; DFNB103; MSTP130
• NCBI Protein Information: chloride intracellular channel protein 5
• UniProt Protein Name: Chloride intracellular channel protein 5
• Protein Family: Chloride intracellular channel protein
• UniProt Gene Name: CLIC5
• UniProt Entry Name: CLIC5_HUMAN

NCBI Description

This gene encodes a member of the chloride intracellular channel (CLIC) family of chloride ion channels. The encoded protein associates with actin-based cytoskeletal structures and may play a role in multiple processes including hair cell stereocilia formation, myoblast proliferation and glomerular podocyte and endothelial cell maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

CLIC5: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Required for normal formation of stereocilia in the inner ear and normal development of the organ of Corti. Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture. Belongs to the chloride channel CLIC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: Golgi apparatus; stereocilium; microtubule organizing center; cell cortex; actin cytoskeleton

Molecular Function: protein binding; chloride channel activity; voltage-gated ion channel activity

Biological Process: diet induced thermogenesis; protein localization; sensory perception of sound; transport; chloride transport; auditory receptor cell stereocilium organization and biogenesis; female pregnancy; neuromuscular process controlling balance

Disease: Deafness, Autosomal Recessive 103