Rabbit MED4 Polyclonal Antibody | anti-MED4 antibody

Anti-MED4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101542

• Gene Names: MED4; ARC36; VDRIP; DRIP36; TRAP36; HSPC126
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: MED4; Polyclonal Antibody; Anti-MED4 Antibody; Rabbit Polyclonal; MED4 Antibody; Rabbit PAb; Antigen Affinity Purified; mediator complex subunit 4; Anti-ARC36 Antibody; Anti-DRIP36 Antibody; Anti-HSPC126 Antibody; Anti-TRAP36 Antibody; Anti-VDRIP Antibody; anti-MED4 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MED4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MED4 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Human MED4
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human MED4, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MED4 rabbit polyclonal antibody at 1:500 dilution Lane A: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:30 kDa Observed band size:35 kDa)

Related Product Information for anti-MED4 antibody

MED4 (Mediator Complex Subunit 4) is a Protein Coding gene. This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. MED4 is widely expressed in testis, bone marrow, and other tissues. Prochlorococcus MED4 has an AT-rich genome, with a GC content of 30.8%, similar to that of Saccharomyces cerevisiae (38%), and contains abundant yeast replication origin consensus sites (ACS) evenly distributed around its 1.66 Mb genome. The MED4 strain is adapted to an oceanic environment characterized by low nutrient levels, and ever-changing light intensities. MED4 the cpeB gene is the only gene coding for a structural phycobiliprotein.

NCBI and Uniprot Product Information

• NCBI GI #: 397739073
• NCBI GeneID: 29079
• NCBI Accession #: NP_001257558.1
• NCBI GenBank Nucleotide #: NM_001270629.1
• UniProt Accession #: Q9NPJ6; Q53GB4; Q53H68; Q5T912; Q6FHC4; Q6IA79; Q9BS95; Q9NYR5; B4DX67
• Molecular Weight: 24,947 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 4 isoform 2
• NCBI Official Synonym Full Names: mediator complex subunit 4
• NCBI Official Symbol: MED4
• NCBI Official Synonym Symbols: ARC36; VDRIP; DRIP36; TRAP36; HSPC126
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 4; TRAP/SMCC/PC2 subunit p36; activator-recruited cofactor 36 kDa component; mediator, 34-kD subunit, homolog; vitamin D receptor-interacting protein, 36-kD; vitamin D3 receptor-interacting protein compl
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 4
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED4
• UniProt Synonym Gene Names: ARC36; DRIP36; VDRIP; ARC36; DRIP36
• UniProt Entry Name: MED4_HUMAN

NCBI Description

This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

MED4: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 4 family.

Chromosomal Location of Human Ortholog: 13q14.2

Cellular Component: nucleoplasm; membrane; Srb-mediator complex; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription cofactor activity; receptor activity; thyroid hormone receptor binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; gene expression