Rabbit MED27 Polyclonal Antibody | anti-MED27 antibody

MED27 Polyclonal Antibody

Cat. Num. AAA3007556

• Gene Names: MED27; MED3; CRSP8; CRAP34; CRSP34; TRAP37
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MED27; Polyclonal Antibody; MED27 Polyclonal Antibody; Mediator of RNA polymerase II transcription subunit 27; Cofactor required for Sp1 transcriptional activation subunit 8; CRSP complex subunit 8; Mediator complex subunit 27; P37 TRAP/SMCC/PC2 subunit; Transcriptional coactivator CRSP34; CRSP34; anti-MED27 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MED27 polyclonal antibody detects endogenous levels of MED27 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 275

• Applicable Applications for anti-MED27 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human MED27.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MED27 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))

Related Product Information for anti-MED27 antibody

In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase. These complexes include CRSP (for cofactor required for Sp1 activation), which is required, in conjunction with TAFIIs, for transcriptional activation by Sp1. CRSP is ubiquitously expressed in various tissues and functions as a multimeric complex that consists of nine distinct subunits. Several members of the CRSP family share sequence similarity with multiple components of the yeast transcriptional mediator proteins, including CRSP150, which is related to yeast Rgr1, and CRSP70, which is similar to the elongation factor TFIIS. CRSP77 and CRSP150 are also related to proteins within the putative murine mediator complex, while CRSP130 and CRSP34 are largely unrelated to either murine or yeast proteins. CRSP subunits also associate with larger multimeric coactivaor complexes, including ARC/DRI, which binds directly to SREBP and nuclear hormone receptors to facilitate transcription, and with NAT, a polymerase II-interacting complex that represses activated transcription

NCBI and Uniprot Product Information

• NCBI GI #: 359751479
• NCBI GeneID: 9442
• NCBI Accession #: NP_001240810.1
• NCBI GenBank Nucleotide #: NP_001240810.1
• UniProt Accession #: Q6P2C8
• Molecular Weight: ~ 35, 31kDa
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 27 isoform 2
• NCBI Official Synonym Full Names: mediator complex subunit 27
• NCBI Official Symbol: MED27
• NCBI Official Synonym Symbols: MED3; CRSP8; CRAP34; CRSP34; TRAP37
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 27
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 27
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED27
• UniProt Synonym Gene Names: CRSP34; CRSP8; CRSP complex subunit 8
• UniProt Entry Name: MED27_HUMAN

NCBI Description

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

Uniprot Description

MED27: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 27 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 9q34.13

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; Srb-mediator complex; nucleus

Molecular Function: protein binding; transcription coactivator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; stem cell maintenance; gene expression