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Western Blot (WB) (Western blot analysis of anti-NFE2L2 Antibody (N-term) in Ramos cell line lysates (35ug/lane). Nrf2(arrow) was detected using the purified Pab.)

Rabbit anti-Human NFE2L2 Polyclonal Antibody | anti-NFE2L2 antibody

NFE2L2, NT (NRF2, Nuclear Factor Erythroid 2-related Factor 2, NFE2-related Factor 2, NF-E2-related Factor 2, Nuclear Factor, Erythroid Derived 2, Like 2, HEBP1) (AP)

Gene Names
RAB3IL1; GRAB
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
NFE2L2; Polyclonal Antibody; NT (NRF2; Nuclear Factor Erythroid 2-related Factor 2; NFE2-related Factor 2; NF-E2-related Factor 2; Nuclear Factor; Erythroid Derived 2; Like 2; HEBP1) (AP); anti-NFE2L2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NRF2.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-NFE2L2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human NRF2 (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-NFE2L2 Antibody (N-term) in Ramos cell line lysates (35ug/lane). Nrf2(arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-NFE2L2 Antibody (N-term) in Ramos cell line lysates (35ug/lane). Nrf2(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of Nrf2(Nfe2l2)(arrow) using rabbit polyclonal Nrf2(Nfe2l2) Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Nrf2(Nfe2l2) gene (Lane 2) (Origene Technologies).)

Western Blot (WB) (Western blot analysis of Nrf2(Nfe2l2)(arrow) using rabbit polyclonal Nrf2(Nfe2l2) Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Nrf2(Nfe2l2) gene (Lane 2) (Origene Technologies).)
Product Categories/Family for anti-NFE2L2 antibody
References
1. Zhao, X., Stroke 38(12), 3280-3286 (2007). 2. Li, M.H., J. Biol. Chem. 282(39), 28577-28586 (2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69.9kDa (625aa)
NCBI Official Full Name
guanine nucleotide exchange factor for Rab-3A isoform 1
NCBI Official Synonym Full Names
RAB3A interacting protein like 1
NCBI Official Symbol
RAB3IL1
NCBI Official Synonym Symbols
GRAB
NCBI Protein Information
guanine nucleotide exchange factor for Rab-3A
UniProt Protein Name
Nuclear factor erythroid 2-related factor 2
UniProt Gene Name
NFE2L2
UniProt Synonym Gene Names
NRF2; NF-E2-related factor 2
UniProt Entry Name
NF2L2_HUMAN

NCBI Description

This gene encodes a guanine nucleotide exchange factor for the ras-related protein Rab3A. The encoded protein binds Rab3a and the inositol hexakisphosphate kinase InsP6K1. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq, Nov 2012]

Uniprot Description

NRF2: a transcription factor that regulates basal expression and antioxidant induction of a transcription factor for NAD(P)H:quinone oxidoreductase-1 (NQO1) and other detoxifying genes. Targeted for proteasomal degradation by INrf2. Oxidative stress causes its phosphorylation and release from INrf2.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: nucleoplasm; centrosome; cytoplasm; plasma membrane; nucleus; chromatin; cytosol

Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; positive regulation of blood coagulation; unfolded protein response; regulation of embryonic development; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter

Research Articles on NFE2L2

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Product Notes

The NFE2L2 nfe2l2 (Catalog #AAA6490628) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NFE2L2, NT (NRF2, Nuclear Factor Erythroid 2-related Factor 2, NFE2-related Factor 2, NF-E2-related Factor 2, Nuclear Factor, Erythroid Derived 2, Like 2, HEBP1) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NFE2L2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NFE2L2 nfe2l2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFE2L2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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