Rabbit anti-Mouse MeCP2 (Ser421) Polyclonal Antibody | anti-MECP2 antibody

Anti-Phospho-Ser421 Methyl-CpG Binding Protein 2 (MeCP2)

Cat. Num. AAA502181

• Gene Names: Mecp2; Mbd5; WBP10; BB130002; 1500041B07Rik; D630021H01Rik
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.)
• Synonyms: MeCP2 (Ser421); Polyclonal Antibody; Anti-Phospho-Ser421 Methyl-CpG Binding Protein 2 (MeCP2); anti-MECP2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: Specific for the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling of the MeCP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.. Adequate amount of material to conduct 10- mini Western Blots
• Sequence Length: 501

• Applicable Applications for anti-MECP2 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser421 of MeCP2 (Methyl-CpG Binding Protein 2) protein.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 conjugated to KLH
• Immunogen Species: Mouse
• Reactivity Assumed Based on 100% Sequence Homology: Human, non-human primate
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with mouse tissue. It is anticipated that the antibody will react with human and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser421. Phosphorylation and dephosphorylation of this site may be involved in regulation of behavioral responses to chronic antidepressant treatment (Hutchinson et al., 2012).
• Preparation and Storage: Store at -20 degree C in undiluted aliquots; stable for at least 1 year after date of receipt. Avoid freeze/thaw cycles.

Testing Data

Related Product Information for anti-MECP2 antibody

Rabbit polyclonal antibody

References

• Amir RE, Van den Veyver IB, Wan M, Tran CQ, Francke U, Zoghbi HY (1999) Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nat Genet. Oct;23(2):185-8.
• Hutchinson AN, Deng JV, Cohen S, West AE (2012) Phosphorylation of MeCP2 at Ser421 contributes to chronic antidepressant action. Journal of Neuroscience. Oct 10;32(41):14355-63.
• Nan X, Ng HH, Johnson CA, Laherty CD, Turner BM, Eisenman RN, Bird A (1998) Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex. Nature. May 28;393(6683):386-9.

NCBI and Uniprot Product Information

• NCBI GI #: 126517469
• NCBI GeneID: 17257
• NCBI Accession #: NP_001075448.1
• NCBI GenBank Nucleotide #: NM_001081979.1
• UniProt Accession #: Q9Z2D6; Q3TYG1; B1AUZ2; B1AUZ3
• Molecular Weight: 52,307 Da
• NCBI Official Full Name: methyl-CpG-binding protein 2 isoform 1
• NCBI Official Synonym Full Names: methyl CpG binding protein 2
• NCBI Official Symbol: Mecp2
• NCBI Official Synonym Symbols: Mbd5; WBP10; BB130002; 1500041B07Rik; D630021H01Rik
• NCBI Protein Information: methyl-CpG-binding protein 2; meCp-2 protein
• UniProt Protein Name: Methyl-CpG-binding protein 2
• Protein Family: Methyl-CpG-binding protein
• UniProt Gene Name: Mecp2
• UniProt Synonym Gene Names: MeCp-2 protein; MeCp2
• UniProt Entry Name: MECP2_MOUSE

Uniprot Description

Function: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). Ref.10

Subunit structure: Interacts with CDKL5

By similarity. Interacts with FNBP3. Ref.7

Subcellular location: Nucleus. Note: Colocalized with methyl-CpG in the genome. Ref.8

Post-translational modification: Phosphorylated on Ser-421 by CaMK2 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. Does not seem to be phosphorylated on Ser-421 in other tissues. Ref.9

Sequence similarities: Contains 2 A.T hook DNA-binding domains.Contains 1 MBD (methyl-CpG-binding) domain.