Testing Data
Rabbit MeCP2 Polyclonal Antibody | anti-MeCP2 antibody
MeCP2 Antibody
Appearance: Colorless liquid
Shelf Life: 12 months
Testing Data
Testing Data
Background: DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. A demethylase enzyme has been identified which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) co-localize with foci of heavily methylated satellite DNA and mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA either in vivo or in vitro. MBD1, MBD2, MBD3, and MBD4 are expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 has homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors, MBD4 is mutated at an exonic polynucleotide tract.
NCBI and Uniprot Product Information
NCBI Description
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009]
Uniprot Description
MECP2: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Interacts with FNBP3. Interacts with CDKL5. Present in all adult somatic tissues tested. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: nucleoplasm; heterochromatin; extracellular space; nucleus; cytosol
Molecular Function: mRNA binding; protein domain specific binding; protein binding; siRNA binding; DNA binding; methyl-CpG binding; double-stranded methylated DNA binding; protein N-terminus binding; chromatin binding; transcription corepressor activity; transcription factor activity; transcription factor binding
Biological Process: catecholamine secretion; startle response; behavioral fear response; genetic imprinting; adult locomotory behavior; positive regulation of transcription, DNA-dependent; chromatin silencing; pathogenesis; neurological control of breathing; proprioception; negative regulation of transcription from RNA polymerase II promoter; sensory perception of pain; negative regulation of histone methylation; neuron maturation; phosphatidylcholine metabolic process; post-embryonic development; negative regulation of histone acetylation; protein localization; synaptogenesis; positive regulation of cell proliferation; dendrite development; visual learning; cerebellum development; histone acetylation; negative regulation of neuron apoptosis; mitochondrial electron transport, ubiquinol to cytochrome c; transcription, DNA-dependent; ventricular system development; glutamine metabolic process; social behavior; respiratory gaseous exchange; cardiolipin metabolic process; histone methylation; positive regulation of synaptogenesis; glucocorticoid metabolic process; regulation of systemic arterial blood pressure by neurological process; long-term memory; inositol metabolic process; response to hypoxia; negative regulation of transcription, DNA-dependent; regulation of excitatory postsynaptic membrane potential
Disease: Rett Syndrome; Mental Retardation, X-linked, Syndromic 13; Autism, Susceptibility To, X-linked 3; Lubs X-linked Mental Retardation Syndrome; Encephalopathy, Neonatal Severe, Due To Mecp2 Mutations; Angelman Syndrome
Research Articles on MeCP2
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Product Notes
The MeCP2 mecp2 (Catalog #AAA841338) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MeCP2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MeCP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blot: 1:200. Researchers should empirically determine the suitability of the MeCP2 mecp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MeCP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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