Western Blot (WB)
((0.3ug/ml) staining of Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat ARK5/NUAK1 Polyclonal Antibody | anti-NUAK1 antibody
Goat anti-ARK5/NUAK1 Antibody
Gene Names
NUAK1; ARK5
Reactivity
Tested: Human; Expected from sequence similarity: Human, Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ARK5/NUAK1; Polyclonal Antibody; Goat anti-ARK5/NUAK1 Antibody; NUAK1; NUAK family; SNF1-like kinase; 1; ARK5; KIAA0537; AMP-activated protein kinase family member 5; AMPK-related protein kinase 5; NUAK1 antibody; 1 antibody; ARK5 antibody; KIAA0537 antibody; AMP-activated protein kinase family member 5 antibody; AMPK-related protein kinase 5 antibody; ARK5 / NUAK1; anti-NUAK1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QNRPRPQYLKRYRNR
Sequence Length
661
Applicable Applications for anti-NUAK1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx.75kDa band observed in Human Kidney, Muscle and Ovary lysates (calculated MW of 74.3kDa according to NP_055655.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot: Approx.75kDa band observed in Human Kidney, Muscle and Ovary lysates (calculated MW of 74.3kDa according to NP_055655.1). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-QNRPRPQYLKRYRNR, from the internal region of the protein sequence according to NP_055655.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,824 Da
NCBI Official Full Name
NUAK family SNF1-like kinase 1
NCBI Official Synonym Full Names
NUAK family kinase 1
NCBI Official Symbol
NUAK1
NCBI Official Synonym Symbols
ARK5
NCBI Protein Information
NUAK family SNF1-like kinase 1
UniProt Protein Name
NUAK family SNF1-like kinase 1
Protein Family
UniProt Gene Name
NUAK1
UniProt Synonym Gene Names
ARK5; KIAA0537; OMPHK1; ARK5
UniProt Entry Name
NUAK1_HUMAN
Uniprot Description
NuaK1: a protein kinase of the CAMKL family. Phosphorylated and activated by AKT1 during glucose starvation. May activate ATM kinase under conditions of nutrient starvation. Suppresses apoptosis induced by nutrient starvation and death receptors via inhibition of caspase 8 activation.
Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; CAMK group; CAMKL family; NuaK subfamily
Chromosomal Location of Human Ortholog: 12q23.3
Cellular Component: cytoplasm; microtubule cytoskeleton; nucleolus; nucleoplasm; nucleus
Molecular Function: ATP binding; metal ion binding; p53 binding; protein binding; protein serine/threonine kinase activity
Biological Process: cell adhesion; protein amino acid phosphorylation; regulation of cell adhesion; regulation of cell proliferation; response to DNA damage stimulus
Research Articles on NUAK1
1. MiR-145 functions as a tumor suppressor targeting NUAK1 in human intrahepatic cholangiocarcinoma.
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Product Notes
The NUAK1 nuak1 (Catalog #AAA421761) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ARK5/NUAK1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ARK5/NUAK1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx.75kDa band observed in Human Kidney, Muscle and Ovary lysates (calculated MW of 74.3kDa according to NP_055655.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the NUAK1 nuak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QNRPRPQYLK RYRNR. It is sometimes possible for the material contained within the vial of "ARK5/NUAK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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