Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ME1 antibody.The lane on the left is treated with the antigen-specific peptide.)
Rabbit ME1 Polyclonal Antibody | anti-ME1 antibody
ME1 Antibody
Gene Names
ME1; MES; HUMNDME
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ME1; Polyclonal Antibody; ME1 Antibody; Cytosolic malic enzyme 1; HUMNDME; Malate dehydrogenase; Malic enzyme 1; NADP(+) dependent; cytosolic; soluble; Malic enzyme; cytoplasmic; MAOX_HUMAN; MES; NADP dependent malic enzyme; NADP ME; NADP-dependent malic enzyme; NADP-ME; Pyruvic malic carboxylase; anti-ME1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ME1 antibody detects endogenous levels of total ME1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
572
Applicable Applications for anti-ME1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm.
Tissue Specificity
Expressed in all tissues tested including liver, placenta and white adipose tissue.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (91%), Rabbit (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ME1 antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ME1 antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ME1 antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ME1 antibody.The lane on the left is treated with the antigen-specific peptide.)
Immunofluorescence (IF)
(MBS9605671 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9605671 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-ME1 antibody
Function: (S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Subunit Structure: Homotetramer.
Similarity: Belongs to the malic enzymes family.
Subunit Structure: Homotetramer.
Similarity: Belongs to the malic enzymes family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 64 kD
Predicted: 65 kDa
Predicted: 65 kDa
NCBI Official Full Name
NADP-dependent malic enzyme
NCBI Official Synonym Full Names
malic enzyme 1
NCBI Official Symbol
ME1
NCBI Official Synonym Symbols
MES; HUMNDME
NCBI Protein Information
NADP-dependent malic enzyme
UniProt Protein Name
NADP-dependent malic enzyme
Protein Family
UniProt Gene Name
ME1
UniProt Synonym Gene Names
NADP-ME
NCBI Description
This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008]
Research Articles on ME1
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Product Notes
The ME1 me1 (Catalog #AAA9605671) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ME1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ME1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the ME1 me1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ME1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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