Western Blot (WB)
(Western blot analysis of extracts of A431 cell and HepG2 cell lines, using PSMC5 antibody.)
Rabbit PSMC5 Polyclonal Antibody | anti-PSMC5 antibody
PSMC5 (Proteasome 26S ATPase Subunit 5, S8, p45, SUG1, SUG-1, TBP10, TRIP1, p45/SUG) (APC)
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of extracts of A431 cell and HepG2 cell lines, using PSMC5 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of A431 cell and HepG2 cell lines, using PSMC5 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophagus using PSMC5 antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophagus using PSMC5 antibody at dilution of 1:200 (400x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF7 cell using PSMC5 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF7 cell using PSMC5 antibody. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Uniprot Description
RPT6: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. Belongs to the AAA ATPase family.
Protein type: Nuclear receptor co-regulator; Proteasome complex; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 17q23.3
Cellular Component: nucleoplasm; proteasome complex; membrane; proteasome regulatory particle; cytoplasm; inclusion body; cytoplasmic vesicle; nucleus; cytosol
Molecular Function: thyrotropin-releasing hormone receptor binding; protein binding; TATA-binding protein binding; ATPase activity; transcription cofactor activity; transcription factor binding; ATP binding
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; positive regulation of transcription, DNA-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; negative regulation of programmed cell death; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
Research Articles on PSMC5
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Product Notes
The PSMC5 psmc5 (Catalog #AAA6433658) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PSMC5 (Proteasome 26S ATPase Subunit 5, S8, p45, SUG1, SUG-1, TBP10, TRIP1, p45/SUG) (APC) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PSMC5 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC). IF: 1:50-1:200 WB: 1:500-1:2000 IHC: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PSMC5 psmc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMC5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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