Typical Testing Data/Standard Curve (for reference only)
Canine NADP-dependent malic enzyme (ME1) ELISA Kit | ME1 elisa kit
Canine NADP-dependent malic enzyme (ME1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: MDA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MDA antibody and an MDA-HRP conjugate. The assay sample and buffer are incubated together with MDA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MDA concentration since MDA from samples and MDA-HRP conjugate compete for the anti-MDA antibody binding site. Since the number of sites is limited, as more sites are occupied by MDA from the sample, fewer sites are left to bind MDA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MDA concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008]
Uniprot Description
ME1: a ubiquitous lipogenic enzyme of the malic enzymes family. Converts (S)-malate + NADP+ ?? pyruvate + CO2 + NADPH. Levels of ME1 and ATP-citrate lyase mRNA, another lipogenic enzyme, are coordinately increased 5-7 fold during the differentiation of 3T3-L1 preadipocytes into adipocytes. Up-regulated by short-term dietary restriction in mice. The cycling of pyruvate by ME1 generates cytosolic NADPH, whereas mitochondrial ME2 responds to elevated amino acids and serves to supply sufficient pyruvate for increased Krebs cycle flux when glucose is limiting.
Protein type: Oxidoreductase; Carbohydrate Metabolism - pyruvate; EC 1.1.1.40
Chromosomal Location of Human Ortholog: 6q12
Cellular Component: mitochondrion; cytosol
Molecular Function: malate dehydrogenase (decarboxylating) activity; malic enzyme activity; electron carrier activity; manganese ion binding; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; NADP binding; ADP binding; NAD binding; oxaloacetate decarboxylase activity
Biological Process: malate metabolic process; carbohydrate metabolic process; response to hormone stimulus; NADP biosynthetic process; cellular lipid metabolic process; response to carbohydrate stimulus; protein tetramerization
Research Articles on ME1
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Product Notes
The Canine ME1 me1 (Catalog #AAA7200810) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7200810 ELISA Kit recognizes Canine ME1. It is sometimes possible for the material contained within the vial of "NADP-dependent malic enzyme (ME1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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