Rabbit MDM2 Polyclonal Antibody | anti-MDM2 antibody

RABBIT ANTI MOUSE MDM2

Cat. Num. AAA222809

• Gene Names: Mdm2; Mdm-2; AA415488; 1700007J15Rik
• Applications: ELISA, Western Blot
• Synonyms: MDM2; Polyclonal Antibody; RABBIT ANTI MOUSE MDM2; anti-MDM2 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects murine Mdm2, a nuclear phosphoprotein that controls intracellular levels of the tumour suppressor protein p53 by ubiquitination and subsequent proteasomal degradation. Mdm2 also interacts with the retinoblastoma (RB) protein and E2F-1, to promote G1-S phase transition in the cell cycle. ; ; Mdm2 activity is controlled by self-ubiquitination; however activation of the PI3K/PKB pathway leads to phosphorylation of Mdm2 at Ser185, which stabilises the protein.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 440

• Applicable Applications for anti-MDM2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 90kDa in mouse MEF cell lysates.; ELISA: Minimum Dilution: 1/3000; Maximum Dilution: 1/12000; Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/2000
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide corresponding to aa 177-195 of mouse Mdm2.; Buffer Solution
• Antiserum Preparation: Antisera to Mdm2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-MDM2 antibody

MBS222809 detects murine Mdm2, a nuclear phosphoprotein that controls intracellular levels of the tumour suppressor protein p53 by ubiquitination and subsequent proteasomal degradation. Mdm2 also interacts with the retinoblastoma (RB) protein and E2F-1, to promote G1-S phase transition in the cell cycle. Mdm2 activity is controlled by self-ubiquitination; however activation of the PI3K/PKB pathway leads to phosphorylation of Mdm2 at Ser185, which stabilises the protein.

NCBI and Uniprot Product Information

• NCBI GI #: 570700841
• NCBI GeneID: 17246
• NCBI Accession #: NP_001275515.1
• NCBI GenBank Nucleotide #: NM_001288586.1
• UniProt Accession #: P23804; Q61040; Q64330; Q91XK7
• Molecular Weight: 49,351 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase Mdm2
• NCBI Official Synonym Full Names: transformed mouse 3T3 cell double minute 2
• NCBI Official Symbol: Mdm2
• NCBI Official Synonym Symbols: Mdm-2; AA415488; 1700007J15Rik
• NCBI Protein Information: E3 ubiquitin-protein ligase Mdm2; double minute 2 protein; oncoprotein Mdm2; p53-binding protein Mdm2
• UniProt Protein Name: E3 ubiquitin-protein ligase Mdm2
• Protein Family: Mdm2-binding protein
• UniProt Gene Name: Mdm2
• UniProt Entry Name: MDM2_MOUSE

Uniprot Description

MDM2: a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 via Akt-mediated MDM2 phosphorylation. Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. Facilitates the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Eight alternatively spliced isoforms have been reported.

Protein type: Ligase; Ubiquitin conjugating system; Ubiquitin ligase; Inhibitor; Oncoprotein; EC 6.3.2.19; Nucleolus; EC 6.3.2.-

Cellular Component: nucleoplasm; nuclear body; protein complex; cytoplasm; plasma membrane; nucleolus; synapse; cytosol; nucleus

Molecular Function: identical protein binding; peroxisome proliferator activated receptor binding; zinc ion binding; p53 binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; protein binding; enzyme binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ubiquitin protein ligase binding; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: traversing start control point of mitotic cell cycle; protein destabilization; protein ubiquitination during ubiquitin-dependent protein catabolic process; positive regulation of cell cycle; protein ubiquitination; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; positive regulation of protein export from nucleus; negative regulation of DNA damage response, signal transduction by p53 class mediator; establishment of protein localization; peptidyl-lysine modification; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein complex assembly; protein catabolic process; regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis