Goat MDFI Polyclonal Antibody | anti-MDFI antibody
Goat anti-MDFI Antibody
Western Blot: Preliminary experiments gave an approx.70kDa band in lysates of cell line HepG2 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25kDa according to NP_005577.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
NCBI and Uniprot Product Information
NCBI Description
This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq, Jul 2008]
Uniprot Description
MDFI: Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways. Belongs to the MDFI family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 6p21
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; transcription factor binding
Biological Process: activation of JNK activity; cytoplasmic sequestering of transcription factor; dorsal/ventral axis specification; embryonic development; embryonic skeletal morphogenesis; negative regulation of DNA binding; negative regulation of transcription from RNA polymerase II promoter; negative regulation of Wnt receptor signaling pathway
Research Articles on MDFI
Similar Products
Product Notes
The MDFI mdfi (Catalog #AAA422186) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MDFI Antibody reacts with Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's MDFI can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Preliminary experiments gave an approx.70kDa band in lysates of cell line HepG2 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25kDa according to NP_005577.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the MDFI mdfi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SKSTTSQIPL QAQED. It is sometimes possible for the material contained within the vial of "MDFI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
