Rabbit anti-Human MDC1 Polyclonal Antibody | anti-MDC1 antibody

MDC1 (Phospho-Ser513) Antibody

Cat. Num. AAA9503379

• Gene Names: MDC1; NFBD1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: MDC1; Polyclonal Antibody; MDC1 (Phospho-Ser513) Antibody; DKFZp781A0122; IER3; KIAA0170; mediator of DNA damage checkpoint 1; MGC166888; NFBD1; anti-MDC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: MDC1 (Phospho-Ser513) Antibody detects endogenous levels of MDC1 only when phosphorylated at Ser513.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1,066

• Applicable Applications for anti-MDC1 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50~1:100; ELISA: 1:1000
• Target Modification: Phospho
• Modification Sites: H:S513
• Immunogen: The antiserum was produced against synthesized peptide derived from human MDC1 around the phosphorylation site of Ser513.
• Immunogen Range: 479-528
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain, using MDC1 (Phospho-Ser513) Antibody. The picture on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 132626688
• NCBI GeneID: 9656
• NCBI Accession #: NP_055456.2
• NCBI GenBank Nucleotide #: NM_014641.2
• UniProt Accession #: Q14676; Q0EFC2; Q2L6H7; Q2TAZ4; Q5JP55; Q5JP56; Q5ST83; A2AB04; A2BF04; A2RRA8; A7YY86; B0S8A2
• Molecular Weight: 226 kDa
• NCBI Official Full Name: mediator of DNA damage checkpoint protein 1
• NCBI Official Synonym Full Names: mediator of DNA damage checkpoint 1
• NCBI Official Symbol: MDC1
• NCBI Official Synonym Symbols: NFBD1
• NCBI Protein Information: mediator of DNA damage checkpoint protein 1
• UniProt Protein Name: Mediator of DNA damage checkpoint protein 1
• Protein Family: Mediator of DNA damage checkpoint protein
• UniProt Gene Name: MDC1
• UniProt Synonym Gene Names: KIAA0170; NFBD1

NCBI Description

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008]

Uniprot Description

Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.