Immunofluorescence (IF)
(Immunofluorescence staining of PRKCB in RT4 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PRKCB polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus and Cytoplasm.)
Rabbit anti-Human PRKCB Polyclonal Antibody | anti-PRKCB antibody
Anti-PRKCB Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
PRKCB; Polyclonal Antibody; Anti-PRKCB Antibody; Rabbit Polyclonal; PRKCB Antibody; Rabbit PAb; Antigen Affinity Purified; protein kinase C; beta; Anti-PKC-beta Antibody; Anti-PKCB Antibody; Anti-PRKCB1 Antibody; Anti-PRKCB2 Antibody; anti-PRKCB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PRKCB
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PRKCB antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human PRKCB fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PRKCB fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of PRKCB in RT4 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PRKCB polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus and Cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of PRKCB in RT4 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PRKCB polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus and Cytoplasm.)
Related Product Information for anti-PRKCB antibody
PRKCB (Protein Kinase C Beta) is a Protein Coding gene. The PRKCB gene, located on 16p12.2-p12.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, S.pombe, and frog. PRKCB was determined to consist of 18 exons covering approximately 375 kb, with a particularly large intron of over 150 kb between exons 2 and 3. PRKCB is nearly 19 times larger than the highly homologous Drosophila melanogaster protein kinase C gene (dPKC), which has a similar-sized open reading frame but only 13 exons. This increase in size has occurred mostly as a result of the expansion of introns, with intron size in the human gene averaging 22 kb compared with 1.5 kb in dPKC. Diseases associated with PRKCB include Diabetic Macular Edema and Microvascular Complications Of Diabetes 5.
Product Categories/Family for anti-PRKCB antibody
References
Zhu Z, et al. (2018) Increased expression of prkcb mrna in peripheral blood mononuclear cells from patients with systemic lupus erythematosus. Ann Hum Genet 82 (4): 200-205.Kawashima M, et al. (2017) Genome-wide association studies identify prkcb as a novel genetic susceptibility locus for primary biliary cholangitis in the japanese population. Hum Mol Genet 26 (3): 650-659.Greenham J, et al. (1998) Elucidation of the exon-intron structure and size of the human protein kinase c beta gene (prkcb). Hum Genet 103 (4): 483-487.
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Product Notes
The PRKCB (Catalog #AAA8109275) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PRKCB Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRKCB can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the PRKCB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKCB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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