Rabbit anti-Human ITIH1 Polyclonal Antibody | anti-ITIH1 antibody

ITIH1, ID (ITIH1, IGHEP1, Inter-alpha-trypsin inhibitor heavy chain H1, Inter-alpha-trypsin inhibitor complex component III, Serum-derived hyaluronan-associated protein) (Azide free) (HRP)

Cat. Num. AAA6311327

• Gene Names: ITIH1; H1P; ITIH; SHAP; IATIH; IGHEP1; ITI-HC1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ITIH1; Polyclonal Antibody; ID (ITIH1; IGHEP1; Inter-alpha-trypsin inhibitor heavy chain H1; Inter-alpha-trypsin inhibitor complex component III; Serum-derived hyaluronan-associated protein) (Azide free) (HRP); Serum-derived hyaluronan-associated protein; anti-ITIH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ITIH1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ITIH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 613-643 amino acids from the Central region of human ITIH1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ITIH1 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ITIH1 antibody detected the ITIH1 protein (arrow).)

Flow Cytometry (FC/FACS)

(ITIH1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ITIH1 antibody

The protein encoded by this gene is the heavy chain of a serine protease inhibitor that may serve to carry hyaluronan in plasma. This gene is part of a cluster of similar genes on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-ITIH1 antibody

Antibodies; Proteins

References

So, H.C., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (1), 103-113 (2010): Scott, L.J., et al. Proc. Natl. Acad. Sci. U.S.A. 106(18):7501-7506(2009). Hamm, A., et al. BMC Cancer 8, 25 (2008): Scarchilli, L., et al. J. Biol. Chem. 282(41):30161-30170(2007). Bunkenborg, J., et al. Proteomics 4(2):454-465(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 156119625
• NCBI GeneID: 3697
• NCBI Accession #: NP_002206.2; NP_001159908.1; NP_001159906.1
• NCBI GenBank Nucleotide #: NM_002215.3
• UniProt Accession #: P19827; P78455; Q01746; Q562G1; A8K9N5; B2RAH9; B7Z558; B7Z8C0; F5H165; F5H7Y8
• Molecular Weight: 69,495 Da
• NCBI Official Full Name: inter-alpha-trypsin inhibitor heavy chain H1 isoform a preproprotein
• NCBI Official Synonym Full Names: inter-alpha-trypsin inhibitor heavy chain 1
• NCBI Official Symbol: ITIH1
• NCBI Official Synonym Symbols: H1P; ITIH; SHAP; IATIH; IGHEP1; ITI-HC1
• NCBI Protein Information: inter-alpha-trypsin inhibitor heavy chain H1
• UniProt Protein Name: Inter-alpha-trypsin inhibitor heavy chain H1
• Protein Family: Inter-alpha-trypsin inhibitor
• UniProt Gene Name: ITIH1
• UniProt Synonym Gene Names: IGHEP1; ITI heavy chain H1; ITI-HC1; Inter-alpha-inhibitor heavy chain 1; SHAP
• UniProt Entry Name: ITIH1_HUMAN

NCBI Description

This gene encodes a member of the inter-alpha-trypsin inhibitor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the heavy chain of the inter-alpha-trypsin inhibitor complex, which is secreted by hepatocytes into the blood. The heavy chain also interacts with hyaluronan, and this interaction may play a role in ovulation and fertilization, and has been implicated in multiple inflammatory diseases. This gene is present in a gene cluster on chromosome 3. [provided by RefSeq, Nov 2015]

Uniprot Description

ITIH1: May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Belongs to the ITIH family.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; calcium ion binding

Biological Process: hyaluronan metabolic process