Rabbit anti-Human MCM10 Polyclonal Antibody | anti-MCM10 antibody

Anti-MCM10 Antibody

Cat. Num. AAA8245889

• Gene Names: MCM10; CNA43; DNA43; PRO2249
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry
• Purity: The antibody was purified by immunogen affinity chromatography.
• Synonyms: MCM10; Polyclonal Antibody; Anti-MCM10 Antibody; Protein MCM10 homolog; HsMCM10; anti-MCM10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of MCM10 protein.
• Purity/Purification: The antibody was purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Sequence Length: 874

• Applicable Applications for anti-MCM10 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB: 1/500 - 1/2000; IF/ICC: 1/50 - 1/100
• Immunogen: Recombinant full length protein of human MCM10
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of MCM10 expression in MCF7 (A), mouse liver (B) whole cell lysates.)

Immunofluorescence (IF)

(Immunofluorescent analysis of MCM10 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

Related Product Information for anti-MCM10 antibody

Rabbit polyclonal antibody to MCM10

NCBI and Uniprot Product Information

• NCBI GI #: 33383235
• NCBI GeneID: 55388
• NCBI Accession #: NP_060988.3
• NCBI GenBank Nucleotide #: NM_018518.4
• UniProt Accession #: Q7L590; Q3MIR3; Q7LD55; Q96GX4; Q96NB6; Q9H0D7; Q9H3P9; Q9P177; A8K9I6; B7ZKZ8; AAB34784; AAB34309
• Molecular Weight: 98,084 Da
• NCBI Official Full Name: protein MCM10 homolog isoform 2
• NCBI Official Synonym Full Names: minichromosome maintenance 10 replication initiation factor
• NCBI Official Symbol: MCM10
• NCBI Official Synonym Symbols: CNA43; DNA43; PRO2249
• NCBI Protein Information: protein MCM10 homolog
• UniProt Protein Name: Protein MCM10 homolog
• Protein Family: Minichromosome maintenance protein
• UniProt Gene Name: MCM10
• UniProt Synonym Gene Names: HsMCM10
• UniProt Entry Name: MCM10_HUMAN

NCBI Description

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

MCM10: Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Belongs to the MCM10 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus

Molecular Function: DNA replication origin binding; enzyme binding; identical protein binding; metal ion binding; protein binding; single-stranded DNA binding

Biological Process: cell proliferation; DNA replication; DNA replication initiation; G1/S transition of mitotic cell cycle; response to DNA damage stimulus