Rabbit anti-Human RASA3 Polyclonal Antibody | anti-RASA3 antibody

RASA3, CT (RASA3, Ras GTPase-activating protein 3, GAP1(IP4BP), Ins P4-binding protein) (Azide free) (HRP)

Cat. Num. AAA6340387

• Gene Names: RASA3; GAPIII; GAP1IP4BP
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RASA3; Polyclonal Antibody; CT (RASA3; Ras GTPase-activating protein 3; GAP1(IP4BP); Ins P4-binding protein) (Azide free) (HRP); Ins P4-binding protein; anti-RASA3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RASA3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RASA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 594-623 amino acids from the C-terminal region of human RASA3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RASA3 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the RASA3 antibody detected the RASA3 protein (arrow).)

Related Product Information for anti-RASA3 antibody

The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This family member is an inositol 1,3,4,5-tetrakisphosphate-binding protein, like the closely related RAS p21 protein activator 2. The two family members have distinct pleckstrin-homology domains, with this particular member having a domain consistent with its localization to the plasma membrane.

Product Categories/Family for anti-RASA3 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Kupzig, S., et al. Mol. Cell. Biol. 29(14):3929-3940(2009). Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005). Walker, S.A., et al. J. Biol. Chem. 277(50):48779-48785(2002). Koehler, J.A., et al. Biochem. Biophys. Res. Commun. 283(4):888-895(2001). Cozier, G.E., et al. J. Biol. Chem. 275(36):28261-28268(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 38201692
• NCBI GeneID: 22821
• NCBI Accession #: NP_031394.2
• NCBI GenBank Nucleotide #: NM_007368.2
• UniProt Accession #: Q14644; Q8IUY2; A6NL15
• Molecular Weight: 95,699 Da
• NCBI Official Full Name: ras GTPase-activating protein 3
• NCBI Official Synonym Full Names: RAS p21 protein activator 3
• NCBI Official Symbol: RASA3
• NCBI Official Synonym Symbols: GAPIII; GAP1IP4BP
• NCBI Protein Information: ras GTPase-activating protein 3; GAP1(IP4BP); ins P4-binding protein; GTPase activating protein III; Ins(1,3,4,5)P4-binding protein; inositol 1,3,4,5-tetrakisphosphate-binding protein
• UniProt Protein Name: Ras GTPase-activating protein 3
• Protein Family: Ras GTPase-activating protein
• UniProt Gene Name: RASA3
• UniProt Entry Name: RASA3_HUMAN

NCBI Description

The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This family member is an inositol 1,3,4,5-tetrakisphosphate-binding protein, like the closely related RAS p21 protein activator 2. The two family members have distinct pleckstrin-homology domains, with this particular member having a domain consistent with its localization to the plasma membrane. [provided by RefSeq, Jul 2008]

Uniprot Description

RASA3: Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4) with high affinity. Might be a specific IP4 receptor.

Protein type: GAPs, Ras; GAPs

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: cytoplasm

Molecular Function: metal ion binding; calcium-release channel activity; GTPase activator activity

Biological Process: negative regulation of Ras protein signal transduction; signal transduction