Rabbit MCL1 / MCL 1 Polyclonal Antibody | anti-MCL1 antibody

Rabbit Polyclonal to Human MCL1 / MCL 1

Cat. Num. AAA240333

• Gene Names: MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Ion exchange Chromatography
• Synonyms: MCL1 / MCL 1; Polyclonal Antibody; Rabbit Polyclonal to Human MCL1 / MCL 1; Anti-MCL1 / MCL 1 Antibody (C-Terminus) IHC-plus; MCL1; BCL2L3; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; Bcl2-L-3; MCL1S; MCL1L; Myeloid cell leukemia ES; MCL 1; MCL1-ES; TM; EAT; Mcl-1; Human MCL1; anti-MCL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: peptide corresponding to 16 amino acids near the carboxy-terminus of human Mcl-1
• Purity/Purification: Ion exchange Chromatography
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 350

• Applicable Applications for anti-MCL1 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: ICC, IHC-P (2.5 ug/ml), WB (1 - 2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human MCL1.
• Immunogen Type: Synthetic peptide
• Immunogen: MCL1 / MCL 1 antibody was raised against synthetic peptide from human MCL1.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-MCL1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of Mcl-1 in Raji cells with Mcl-1 antibody at 10 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry staining of Raji cells using Mcl-1 antibody at 2 ug/ml.)

Western Blot (WB)

(Western blot of Mcl-1 in Raji cell lysates with Mcl-1 antibody at (A) 1 and (B) 2 ug/ml.)

Related Product Information for anti-MCL1 antibody

Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types.

NCBI and Uniprot Product Information

• NCBI GI #: 11386165
• NCBI GeneID: 4170
• NCBI Accession #: NP_068779.1
• NCBI GenBank Nucleotide #: NM_021960.4
• UniProt Accession #: Q07820; Q9HD91; Q9NRQ3; Q9NRQ4; Q9UHR7; Q9UHR8; Q9UHR9; Q9UNJ1; B2R6B2; D3DV03; D3DV04
• Molecular Weight: 28,662 Da
• NCBI Official Full Name: induced myeloid leukemia cell differentiation protein Mcl-1 isoform 1
• NCBI Official Synonym Full Names: myeloid cell leukemia 1
• NCBI Official Symbol: MCL1
• NCBI Official Synonym Symbols: TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• NCBI Protein Information: induced myeloid leukemia cell differentiation protein Mcl-1; bcl-2-like protein 3; myeloid cell leukemia ES; bcl-2-related protein EAT/mcl1; myeloid cell leukemia sequence 1 (BCL2-related)
• UniProt Protein Name: Induced myeloid leukemia cell differentiation protein Mcl-1
• Protein Family: Minichromosome loss protein
• UniProt Gene Name: MCL1
• UniProt Synonym Gene Names: BCL2L3; Bcl2-L-3
• UniProt Entry Name: MCL1_HUMAN

NCBI Description

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

Uniprot Description

MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

Protein type: Channel, misc.; Mitochondrial; Apoptosis; Membrane protein, integral; Inhibitor

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; mitochondrial outer membrane; membrane; mitochondrion; mitochondrial matrix; cytoplasm; integral to membrane; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding

Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; multicellular organismal development; cellular homeostasis; cell fate determination