Mouse anti-Human MCL1 / MCL 1 Monoclonal Antibody | anti-MCL1 antibody

Mouse Monoclonal [clone 2E11] (IgG2a) to Human MCL1 / MCL 1

Cat. Num. AAA249129

• Gene Names: MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A/G Purified
• Synonyms: MCL1 / MCL 1; Monoclonal Antibody; Mouse Monoclonal [clone 2E11] (IgG2a) to Human MCL1 / MCL 1; Anti-MCL1 / MCL 1 Antibody (clone 2E11) IHC-plus; MCL1; BCL2L3; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; Bcl2-L-3; MCL1S; MCL1L; Myeloid cell leukemia ES; MCL 1; MCL1-ES; TM; EAT; Mcl-1; Human MCL1; anti-MCL1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 2E11
• Specificity: Human MCL 1
• Purity/Purification: Protein A/G Purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 0.89 mg/ml (varies by lot)
• Sequence Length: 350

• Applicable Applications for anti-MCL1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo (1:100), IF (1:100), IHC-P (1:50, 10 ug/ml), WB (1:2000); Usage: Concentration: 0.5-1.0 mg/ml (Lot dependent)
• Target Species: Human
• Immunogen Description: Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Immunogen: MCL1 / MCL 1 antibody was raised against full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MCL1 / MCL 1 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescent staining of HepG2 cells using anti-MCL1 mouse monoclonal antibody.)

Immunofluorescence (IF)

(Anti-MCL1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1.)

Flow Cytometry (FC/FACS)

(HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal, and then analyzed by flow cytometry.)

Flow Cytometry (FC/FACS)

(Flow cytometry of HeLa cells, using anti-MCL1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).)

Flow Cytometry (FC/FACS)

(Flow cytometry of Jurkat cells, using anti-MCL1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.)

Related Product Information for anti-MCL1 antibody

Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types.

NCBI and Uniprot Product Information

• NCBI GI #: 11386165
• NCBI GeneID: 4170
• NCBI Accession #: NP_068779.1
• NCBI GenBank Nucleotide #: NM_021960.4
• UniProt Accession #: Q07820; Q9HD91; Q9NRQ3; Q9NRQ4; Q9UHR7; Q9UHR8; Q9UHR9; Q9UNJ1; B2R6B2; D3DV03; D3DV04
• Molecular Weight: 28,662 Da
• NCBI Official Full Name: induced myeloid leukemia cell differentiation protein Mcl-1 isoform 1
• NCBI Official Synonym Full Names: myeloid cell leukemia 1
• NCBI Official Symbol: MCL1
• NCBI Official Synonym Symbols: TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• NCBI Protein Information: induced myeloid leukemia cell differentiation protein Mcl-1; bcl-2-like protein 3; myeloid cell leukemia ES; bcl-2-related protein EAT/mcl1; myeloid cell leukemia sequence 1 (BCL2-related)
• UniProt Protein Name: Induced myeloid leukemia cell differentiation protein Mcl-1
• Protein Family: Minichromosome loss protein
• UniProt Gene Name: MCL1
• UniProt Synonym Gene Names: BCL2L3; Bcl2-L-3
• UniProt Entry Name: MCL1_HUMAN

NCBI Description

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

Uniprot Description

MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

Protein type: Channel, misc.; Inhibitor; Mitochondrial; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding

Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; multicellular organismal development; response to cytokine stimulus; cell fate determination; cellular homeostasis