Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using MCHR2 antibody.)
Rabbit anti-Human MCHR2 Polyclonal Antibody | anti-MCHR2 antibody
MCHR2 Antibody
Gene Names
MCHR2; SLT; MCH2; MCH2R; GPR145; GPRv17; MCH-2R; MCH-R2; MCHR-2
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MCHR2; Polyclonal Antibody; MCHR2 Antibody; G protein coupled receptor 145; G protein coupled receptor slt; G-protein coupled receptor 145; GPR145; GPRv17; MCH receptor 2; MCH-2R; MCH-R2; MCH2; MCH2R; MCHR-2; MCHR2_HUMAN; Melanin concentrating hormone receptor 2; Melanin-concentrating hormone receptor 2; SLT; anti-MCHR2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
MCHR2 antibody detects endogenous levels of total MCHR2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
340
Applicable Applications for anti-MCHR2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane
Tissue Specificity
Specifically expressed in the brain, with highest levels in cerebral cortex, hippocampus and amygdala. No expression detected in the cerebellum, thalamus or hypothalamus.
Predicted Cross Reactivity
Bovine
Similarity
Bovine (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using MCHR2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using MCHR2 antibody.)
Immunofluorescene (IF)
(MBS9606438 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9606438 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-MCHR2 antibody
Function: Receptor for melanin-concentrating hormone, coupled to G proteins that activate phosphoinositide hydrolysis.
Similarity: Belongs to the G-protein coupled receptor 1 family.
Similarity: Belongs to the G-protein coupled receptor 1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 39 kD
Predicted: 39 kDa
Predicted: 39 kDa
NCBI Official Full Name
melanin-concentrating hormone receptor 2
NCBI Official Synonym Full Names
melanin concentrating hormone receptor 2
NCBI Official Symbol
MCHR2
NCBI Official Synonym Symbols
SLT; MCH2; MCH2R; GPR145; GPRv17; MCH-2R; MCH-R2; MCHR-2
NCBI Protein Information
melanin-concentrating hormone receptor 2
UniProt Protein Name
Melanin-concentrating hormone receptor 2
Protein Family
UniProt Gene Name
MCHR2
UniProt Synonym Gene Names
GPR145; SLT; MCH receptor 2; MCH-R2; MCHR-2; MCH2; MCH2R
Uniprot Description
Receptor for melanin-concentrating hormone, coupled to G proteins that activate phosphoinositide hydrolysis.
Research Articles on MCHR2
Similar Products
Product Notes
The MCHR2 mchr2 (Catalog #AAA9606438) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MCHR2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MCHR2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the MCHR2 mchr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCHR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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