Rabbit anti-Human MCHR1 Polyclonal Antibody | anti-MCHR1 antibody

MCHR1, CT (MCHR1, GPR24, SLC1, Melanin-concentrating hormone receptor 1, G-protein coupled receptor 24, MCH-1R, SLC-1, Somatostatin receptor-like protein) (MaxLight 750)

Cat. Num. AAA6319637

• Gene Names: MCHR1; SLC1; GPR24; MCH1R; SLC-1; MCH-1R
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MCHR1; Polyclonal Antibody; CT (MCHR1; GPR24; SLC1; Melanin-concentrating hormone receptor 1; G-protein coupled receptor 24; MCH-1R; SLC-1; Somatostatin receptor-like protein) (MaxLight 750); Somatostatin receptor-like protein; anti-MCHR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MCHR1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-MCHR1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 396-422 in the C-terminal region of human MCHR1, UniProt Accession #Q99705. Species sequence homology: mouse and rat
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue with MBS648282 which was peroxidase conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6001583 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of K562 cells using MBS648282 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western Blot analysis in CEM,K562 cell line lysates (35ug/lane) usingMBS6001583. This demonstrates that MBS648282 detected the MCHR1 protein (arrow).)

Related Product Information for anti-MCHR1 antibody

The protein encoded by this gene, a member of the G protein-coupled receptor family 1, is an integral plasma membrane protein which binds melanin-concentrating hormone. The encoded protein can inhibit cAMP accumulation and stimulate intracellular calcium flux, and is probably involved in the neuronal regulation of food consumption. Although structurally similar to somatostatin receptors, this protein does not seem to bind somatostatin.

Product Categories/Family for anti-MCHR1 antibody

Antibodies; G Protein Receptors

References

1. Miyamoto-Matsubara, M., et al. Ann. N. Y. Acad. Sci. 1200, 112-119 (2010). 2. Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009). 3. Miller, C.L., et al. Schizophr. Res. 113 (2-3), 259-267 (2009). 4. Gavalas, N.G., et al. Exp. Dermatol. 18(5):454-463(2009). 5. de Krom, M., et al. Biol. Psychiatry 65(7):625-630(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 88758590
• NCBI GeneID: 2847
• NCBI Accession #: NP_005288.3
• NCBI GenBank Nucleotide #: NM_005297.3
• UniProt Accession #: Q99705; Q5R3J1; Q96S47; Q9BV08; B2RBX6
• Molecular Weight: 45,963 Da
• NCBI Official Full Name: melanin-concentrating hormone receptor 1
• NCBI Official Synonym Full Names: melanin-concentrating hormone receptor 1
• NCBI Official Symbol: MCHR1
• NCBI Official Synonym Symbols: SLC1; GPR24; MCH1R; SLC-1; MCH-1R
• NCBI Protein Information: melanin-concentrating hormone receptor 1; MCH receptor 1; G protein-coupled receptor 24; somatostatin receptor-like protein
• UniProt Protein Name: Melanin-concentrating hormone receptor 1
• Protein Family: Melanin-concentrating hormone receptor
• UniProt Gene Name: MCHR1
• UniProt Synonym Gene Names: GPR24; SLC1; MCH receptor 1; MCH-R1; MCHR-1; MCH1R; MCHR
• UniProt Entry Name: MCHR1_HUMAN

NCBI Description

The protein encoded by this gene, a member of the G protein-coupled receptor family 1, is an integral plasma membrane protein which binds melanin-concentrating hormone. The encoded protein can inhibit cAMP accumulation and stimulate intracellular calcium flux, and is probably involved in the neuronal regulation of food consumption. Although structurally similar to somatostatin receptors, this protein does not seem to bind somatostatin. [provided by RefSeq, Jul 2008]

Uniprot Description

MCHR1: Receptor for melanin-concentrating hormone, coupled to both G proteins that inhibit adenylyl cyclase and G proteins that activate phosphoinositide hydrolysis. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: integral to plasma membrane; nonmotile primary cilium; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein C-terminus binding; neuropeptide receptor activity; hormone binding; melanin-concentrating hormone receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; generation of precursor metabolites and energy; neuropeptide signaling pathway; feeding behavior; G-protein signaling, adenylate cyclase inhibiting pathway; positive regulation of calcium ion transport