Western Blot (WB)
(Western blot analysis of GEF H1 on 293T and A431 cells lysates using anti-GEF H1 antibody at 1/500 dilution.)
Rabbit GEF H1 Monoclonal Antibody | anti-GEFH1 antibody
GEF H1 Antibody
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Western Blot (WB)
(Western blot analysis of GEF H1 on 293T and A431 cells lysates using anti-GEF H1 antibody at 1/500 dilution.)
Western Blot (WB)
(Western blot analysis of GEF H1 on 293T and A431 cells lysates using anti-GEF H1 antibody at 1/500 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining GEF H1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining GEF H1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining GEF H1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining GEF H1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HL-60 cells with GEF H1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HL-60 cells with GEF H1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]
Uniprot Description
ARHGEF2: a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases. Mediates cross-talk between microtubules and the actin cytoskeleton. Contains a phorbol esters/diacylglycerol binding and PH domain.
Protein type: GEFs, Rac/Rho; GEFs; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1q21-q22
Cellular Component: Golgi apparatus; microtubule; protein complex; tight junction; cytoskeleton; focal adhesion; cell soma; cytoplasmic membrane-bound vesicle; cytoplasm; spindle; dendritic shaft; cytosol; vesicle
Molecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; Rho GTPase binding; zinc ion binding; Rac guanyl-nucleotide exchange factor activity; microtubule binding; Rac GTPase binding; transcription factor binding
Biological Process: mitosis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; negative regulation of microtubule depolymerization; cell morphogenesis; positive regulation of interleukin-6 production; actin filament organization; positive regulation of tumor necrosis factor production; regulation of cell proliferation; negative regulation of neurogenesis; activation of NF-kappaB transcription factor; intracellular protein transport; regulation of small GTPase mediated signal transduction; positive regulation of peptidyl-tyrosine phosphorylation; establishment of mitotic spindle orientation; cell division; small GTPase mediated signal transduction; regulation of Rho protein signal transduction; innate immune response; positive regulation of transcription from RNA polymerase II promoter
Research Articles on GEFH1
Similar Products
Product Notes
The GEFH1 arhgef2 (Catalog #AAA9436642) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GEF H1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GEF H1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the GEFH1 arhgef2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GEF H1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
