Rabbit anti-Human MCF2L Polyclonal Antibody | anti-MCF2L antibody

MCF2L, CT (MCF2L, KIAA0362, Guanine nucleotide exchange factor DBS, DBL's big sister, MCF2-transforming sequence-like protein) (Biotin)

Cat. Num. AAA6319619

• Gene Names: MCF2L; DBS; OST; ARHGEF14
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MCF2L; Polyclonal Antibody; CT (MCF2L; KIAA0362; Guanine nucleotide exchange factor DBS; DBL's big sister; MCF2-transforming sequence-like protein) (Biotin); MCF2-transforming sequence-like protein; anti-MCF2L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MCF2L antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MCF2L antibody is generated from rabbits immunized with a KLH conjugated synthetic between 1048-1075 amino acids selected from the C-terminal region of human MCF2L.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MCF2L Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MCF2L antibody detected the MCF2L protein (arrow).)

Related Product Information for anti-MCF2L antibody

Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42.

Product Categories/Family for anti-MCF2L antibody

Antibodies; KIAA; orf

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010). Ieguchi, K., et al. J. Biol. Chem. 282(32):23296-23305(2007). Yang, Q., et al. BMC Med. Genet. 8 SUPPL 1, S12 (2007): Kostenko, E.V., et al. J. Biol. Chem. 280(4):2807-2817(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 163644325
• NCBI GeneID: 23263
• NCBI Accession #: NP_001106203.1; NP_079255.3
• NCBI GenBank Nucleotide #: NM_001112732.1
• UniProt Accession #: O15068; Q5JU56; Q5VXT1; Q6ZWD4; Q765G8; A2A2X1; A2A2X2; A2A3G6; A2A3G8; B4DHD6; B4DIL6; E9PDN8
• Molecular Weight: 126,111 Da
• NCBI Official Full Name: guanine nucleotide exchange factor DBS isoform a
• NCBI Official Synonym Full Names: MCF.2 cell line derived transforming sequence like
• NCBI Official Symbol: MCF2L
• NCBI Official Synonym Symbols: DBS; OST; ARHGEF14
• NCBI Protein Information: guanine nucleotide exchange factor DBS
• UniProt Protein Name: Guanine nucleotide exchange factor DBS
• Protein Family: Guanine nucleotide exchange factor
• UniProt Gene Name: MCF2L
• UniProt Synonym Gene Names: KIAA0362
• UniProt Entry Name: MCF2L_HUMAN

NCBI Description

This gene encodes a guanine nucleotide exchange factor that interacts specifically with the GTP-bound Rac1 and plays a role in the Rho/Rac signaling pathways. A variant in this gene was associated with osteoarthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

ARHGEF14: Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus. Isoform 5 activates CDC42. Belongs to the MCF2 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; GEFs, Rac/Rho; GEFs

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: extracellular space; lamellipodium; plasma membrane; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity; phosphatidylinositol binding

Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; positive regulation of transcription from RNA polymerase II promoter; Rho protein signal transduction