Western Blot (WB)
(Western blot analysis of MCF2 expression in K562 cell line lysate ;, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse MCF2 Polyclonal Antibody | anti-MCF2 antibody
MCF2 Antibody
Gene Names
MCF2; DBL; ARHGEF21
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MCF2; Polyclonal Antibody; MCF2 Antibody; DBL; DBL-transforming protein; MCF2-transforming protein; Proto-oncogene DBL; Proto-oncogene MCF-2; anti-MCF2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
MCF2 antibody detects endogenous levels of total MCF2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
985
Applicable Applications for anti-MCF2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm Isoform 1: Membrane Isoform 3: Membrane. Note: Colocalizes with CDC42 to plasma membrane.
Tissue Specificity
Isoform 1 is expressed only in brain. Isoform 3 is expressed in heart, kidney, spleen, liver and testis. Isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach and peripheral blood. The protein is detectable in brain, heart, kidney, intestine, muscle, lung and testis.
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of MCF2 expression in K562 cell line lysate ;, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of MCF2 expression in K562 cell line lysate ;, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-MCF2 antibody
Function: Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42.
Subunit Structure: Interacts with an array of inositol phospholipids such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P). May interact with CCPG1.
Post-translational Modifications: Phosphorylation by TNK2 enhances guanine nucleotide exchange factor (GEF) activity toward Rho family proteins.
Similarity: The CRAL-TRIO domain is involved in interaction with inositol phospholipids.The DH domain is essential for transforming activity and directly catalyzes GDP-GTP exchange activity. It may interact with CCPG1. Belongs to the MCF2 family.
Subunit Structure: Interacts with an array of inositol phospholipids such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P). May interact with CCPG1.
Post-translational Modifications: Phosphorylation by TNK2 enhances guanine nucleotide exchange factor (GEF) activity toward Rho family proteins.
Similarity: The CRAL-TRIO domain is involved in interaction with inositol phospholipids.The DH domain is essential for transforming activity and directly catalyzes GDP-GTP exchange activity. It may interact with CCPG1. Belongs to the MCF2 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 108 kDa
Predicted: 108 kDa
Predicted: 108 kDa
NCBI Official Full Name
proto-oncogene DBL isoform a
NCBI Official Synonym Full Names
MCF.2 cell line derived transforming sequence
NCBI Official Symbol
MCF2
NCBI Official Synonym Symbols
DBL; ARHGEF21
NCBI Protein Information
proto-oncogene DBL
UniProt Protein Name
Proto-oncogene DBL
Protein Family
UniProt Gene Name
MCF2
UniProt Synonym Gene Names
DBL
NCBI Description
The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.[provided by RefSeq, Jan 2010]
Uniprot Description
Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42.
Research Articles on MCF2
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Product Notes
The MCF2 mcf2 (Catalog #AAA9604074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MCF2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MCF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the MCF2 mcf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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