Typical Testing Data/Standard Curve (for reference only)
Human Vasoactive Intestinal Peptide, VIP ELISA Kit | VIP elisa kit
Human Vasoactive Intestinal Peptide, VIP ELISA Kit
Gene Names
VIP; PHM27
Reactivity
Human
Synonyms
Vasoactive Intestinal Peptide; VIP; Human Vasoactive Intestinal Peptide; VIP ELISA Kit; VIP elisa kit
Reactivity
Human
Sequence Length
121
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
2pg/ml - 600pg/ml
Sensitivity
1.01pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for VIP elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Vasoactive Intestinal Peptide (also known as VIP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human VIP antibody. VIP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human VIP Antibody is added and binds to VIP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VIP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human VIP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human VIP antibody. VIP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human VIP Antibody is added and binds to VIP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VIP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human VIP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
19,082 Da
NCBI Official Full Name
vasoactive intestinal peptide, partial
NCBI Official Synonym Full Names
vasoactive intestinal peptide
NCBI Official Symbol
VIP
NCBI Official Synonym Symbols
PHM27
NCBI Protein Information
VIP peptides
UniProt Protein Name
VIP peptides
Protein Family
UniProt Gene Name
VIP
UniProt Synonym Gene Names
VIP
NCBI Description
The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2014]
Uniprot Description
VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.
Research Articles on VIP
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Product Notes
The Human VIP vip (Catalog #AAA161476) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA161476 ELISA Kit recognizes Human VIP. It is sometimes possible for the material contained within the vial of "Vasoactive Intestinal Peptide, VIP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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