Mouse FBXO32 ELISA Kit | FBXO32 elisa kit

Mouse FBXO32 (F-Box Protein 32) ELISA Kit

Cat. Num. AAA2501718

• Gene Names: FBXO32; Fbx32; MAFbx
• Reactivity: Mouse
• Synonyms: FBXO32; Mouse FBXO32 (F-Box Protein 32) ELISA Kit; FBXO32 elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant Mouse FBXO32. No significant cross-reactivity or interference between Mouse FBXO32 and analogues was observed.
• Sequence Length: 262

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 39.063-2500pg/mL
• Sensitivity: Min: 23.438pg/mL; Max: 2500pg/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse FBXO32 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBXO32 elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FBXO32. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FBXO32 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FBXO32, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FBXO32. You can calculate the concentration of FBXO32 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 335057520
• NCBI GeneID: 114907
• NCBI Accession #: NP_001229392.1
• NCBI GenBank Nucleotide #: NM_001242463.1
• UniProt Accession #: Q969P5; A4KYM0
• Molecular Weight: 30,804 Da
• NCBI Official Full Name: F-box only protein 32 isoform 3
• NCBI Official Synonym Full Names: F-box protein 32
• NCBI Official Symbol: FBXO32
• NCBI Official Synonym Symbols: Fbx32; MAFbx
• NCBI Protein Information: F-box only protein 32; atrogin 1; atrogin-1; muscle atrophy F-box protein
• UniProt Protein Name: F-box only protein 32
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO32
• UniProt Synonym Gene Names: MAFbx
• UniProt Entry Name: FBX32_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Uniprot Description

FBXO32: Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: nucleolus; Z disc

Molecular Function: protein binding

Biological Process: protein ubiquitination; response to denervation involved in regulation of muscle adaptation