Rabbit MBP tag Polyclonal Antibody | anti-malE antibody

MBP tag Antibody

Cat. Num. AAA9217333

• Gene Names: malE; ECK4026; JW3994; malJ
• Applications: Western Blot, ELISA
• Synonyms: MBP tag; Polyclonal Antibody; MBP tag Antibody; Maltose-binding periplasmic protein; MBP; MMBP; Maltodextrin-binding protein; malE; Purified Rabbit Polyclonal Antibody (Pab); anti-malE antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This MBP tag antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide of MBP tag.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.69 (varies by lot)
• Sequence Length: 396

• Applicable Applications for anti-malE antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Cellular Location: Periplasm.
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-MBP tag Antibody at 1:16000 dilution + E. coli lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-MBP tag Antibody at 1:2000 dilution + Recombinant MBP MART1 lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-malE antibody

Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.

References

Duplay P.,et al.J. Biol. Chem. 259:10606-10613(1984). Blattner F.R.,et al.Nucleic Acids Res. 21:5408-5417(1993). Blattner F.R.,et al.Science 277:1453-1462(1997). Hayashi K.,et al.Mol. Syst. Biol. 2:E1-E5(2006). Bedouelle H.,et al.Mol. Gen. Genet. 185:82-87(1982).

NCBI and Uniprot Product Information

• NCBI GI #: 84027873
• NCBI GeneID: 948538
• UniProt Accession #: P0AEX9; P02928; Q2M6S0
• Molecular Weight: 43388 Da
• NCBI Official Full Name: Maltose-binding periplasmic protein
• NCBI Official Symbol: malE
• NCBI Official Synonym Symbols: ECK4026; JW3994; malJ
• NCBI Protein Information: maltose transporter subunit
• UniProt Protein Name: Maltose-binding periplasmic protein
• Protein Family: Maltotriose-binding protein
• UniProt Gene Name: malE
• UniProt Entry Name: MALE_ECOLI

NCBI Description

Biofilm microarray result was validated with RT-PCR. [More information is available at EcoGene: EG10554]. In addition to being the periplasmic substrate-binding component of the maltose ABC transporter, MalE with bound substrate is also able to bind to the chemoreceptor Tar to induce chemotaxis toward maltose. [More information is available at EcoCyc: EG10554].

Uniprot Description

maltose-binding protein: Involved in the high-affinity maltose membrane transport system malEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides

Protein type: Transporter