Western Blot (WB)
(Western blot analysis of Maltose Binding Protein on recombinant MBP-tag protein lysates using anti-Maltose Binding Protein antibody at 1/1, 000 dilution.)
Rabbit anti-E Coli Maltose Binding Protein Monoclonal Antibody | anti-malE antibody
Maltose Binding Protein Antibody
Gene Names
malE; ECK4026; JW3994; malJ
Reactivity
E Coli
Applications
Western Blot
Purity
ProA affinity purified
Synonyms
Maltose Binding Protein; Monoclonal Antibody; Maltose Binding Protein Antibody; ECK4026 antibody; JW3994 antibody; Mal E antibody; MalE antibody; malJ antibody; Maltodextrin binding protein antibody; Maltose ABC transporter periplasmic protein antibody; Maltose binding periplasmic protein antibody; MMBP antibody; Periplasmic maltose binding protein antibody; anti-malE antibody
Host
Rabbit
Reactivity
E Coli
Clonality
Monoclonal
Clone Number
SR41-04
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-malE antibody
Western Blot (WB)
Application Notes
WB: 1:1000-5000
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of Maltose Binding Protein on recombinant MBP-tag protein lysates using anti-Maltose Binding Protein antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of Maltose Binding Protein on recombinant MBP-tag protein lysates using anti-Maltose Binding Protein antibody at 1/1, 000 dilution.)
Related Product Information for anti-malE antibody
Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors.
Product Categories/Family for anti-malE antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,388 Da
NCBI Official Full Name
maltose transporter subunit
NCBI Official Symbol
malE
NCBI Official Synonym Symbols
ECK4026; JW3994; malJ
NCBI Protein Information
maltose transporter subunit
UniProt Protein Name
Maltose-binding periplasmic protein
Protein Family
UniProt Gene Name
malE
NCBI Description
Biofilm microarray result was validated with RT-PCR. [More information is available at EcoGene: EG10554]. In addition to being the periplasmic substrate-binding component of the maltose ABC transporter, MalE with bound substrate is also able to bind to the chemoreceptor Tar to induce chemotaxis toward maltose. [More information is available at EcoCyc: EG10554].
Uniprot Description
maltose-binding protein: Involved in the high-affinity maltose membrane transport system malEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides
Protein type: Transporter
Cellular Component: ATP-binding cassette (ABC) transporter complex; outer membrane-bounded periplasmic space; periplasmic space
Molecular Function: protein binding; transporter activity
Biological Process: carbohydrate transport; maltodextrin transport; maltose transport; response to DNA damage stimulus; transport
Research Articles on malE
Similar Products
Product Notes
The malE male (Catalog #AAA9435508) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Maltose Binding Protein Antibody reacts with E Coli and may cross-react with other species as described in the data sheet. AAA Biotech's Maltose Binding Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000-5000. Researchers should empirically determine the suitability of the malE male for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Maltose Binding Protein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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