Western Blot (WB)
(Western Blot: Sample: Mouse Marrow Tissue.)
Rabbit anti-Human Matrix Metalloproteinase 9 (MMP9) Polyclonal Antibody | anti-MMP9 antibody
Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GKGVVVPT RFGNADGAAC HFPFIFEGRSYSACTTDGRS DGLPWCSTTA NYDTDDRFGF CPSERLYTQD GNADGKPCQF PFIFQGQSYS ACTTDGRSDG YRWCATTANY DRDKLFGFCP TRADSTVMGG NSAGELCVFP
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Mouse Marrow Tissue.)
Western Blot (WB)
(Western Blot: Sample: Mouse Marrow Tissue.)
Western Blot (WB)
(Western Blot: Sample: RecombinantMMP9,Human.)
Western Blot (WB)
(Western Blot: Sample: RecombinantMMP9,Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Lung Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Lung Cancer Tissue.)
NCBI and Uniprot Product Information
NCBI Description
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]
Uniprot Description
MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family.
Protein type: EC 3.4.24.35; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 20q13.12
Cellular Component: extracellular exosome; extracellular region; extracellular space; ficolin-1-rich granule lumen; proteinaceous extracellular matrix; tertiary granule lumen
Molecular Function: collagen binding; endopeptidase activity; identical protein binding; metalloendopeptidase activity; metallopeptidase activity; protein binding; serine-type endopeptidase activity; zinc ion binding
Biological Process: cellular response to cadmium ion; cellular response to reactive oxygen species; collagen catabolic process; embryo implantation; endodermal cell differentiation; ephrin receptor signaling pathway; extracellular matrix disassembly; leukocyte migration; macrophage differentiation; negative regulation of apoptosis; negative regulation of cation channel activity; negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway; negative regulation of intrinsic apoptotic signaling pathway; neutrophil degranulation; ossification; positive regulation of cell migration; positive regulation of DNA binding; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; positive regulation of protein phosphorylation; positive regulation of receptor binding; positive regulation of release of cytochrome c from mitochondria; positive regulation of vascular smooth muscle cell proliferation; proteolysis; skeletal system development
Disease: Metaphyseal Anadysplasia 2
Research Articles on MMP9
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Product Notes
The MMP9 mmp9 (Catalog #AAA2003167) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Matrix Metalloproteinase 9 (MMP9) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the MMP9 mmp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GKGVVVPT RFGNADGAAC HFPFIFEGRS YSACTTDGRS DGLPWCSTTA NYDTDDRFGF CPSERLYTQD GNADGKPCQF PFIFQGQSYS ACTTDGRSDG YRWCATTANY DRDKLFGFCP TRADSTVMGG NSAGELCVFP. It is sometimes possible for the material contained within the vial of "Matrix Metalloproteinase 9 (MMP9), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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