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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Rat Matrix Gla Protein (MGP) Polyclonal Antibody | anti-MGP antibody

Polyclonal Antibody to Matrix Gla Protein (MGP)

Gene Names
Mgp; Mglap
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Matrix Gla Protein (MGP); Polyclonal Antibody; Polyclonal Antibody to Matrix Gla Protein (MGP); anti-MGP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MGP. It has been selected for its ability to recognize MGP in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-MGP antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-5000
Optimal working dilutions must be determined by end user.
Immunogen
MGP, Rat
Immunogen Information
Recombinant MGP (Tyr20~Lys103) with two N-terminal Tags, His-tag and S-tag expressed in E.coli.
Accession No.
RPB477Ra01
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2055632
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:RatTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:RatTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,037 Da
NCBI Official Full Name
matrix Gla protein
NCBI Official Synonym Full Names
matrix Gla protein
NCBI Official Symbol
Mgp
NCBI Official Synonym Symbols
Mglap
NCBI Protein Information
matrix Gla protein
UniProt Protein Name
Matrix Gla protein
Protein Family
UniProt Gene Name
Mgp
UniProt Synonym Gene Names
MGP

NCBI Description

a vitamin K-dependent protein; part of the bone matrix [RGD, Feb 2006]

Uniprot Description

MGP: Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation. Defects in MGP are the cause of Keutel syndrome (KS). KS is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia. Belongs to the osteocalcin/matrix Gla protein family.

Protein type: Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q43

Cellular Component: endoplasmic reticulum; extracellular exosome; extracellular matrix; extracellular space; protein complex

Molecular Function: calcium ion binding; calcium-dependent protein binding; extracellular matrix structural constituent

Biological Process: branching morphogenesis of a tube; cartilage development; cell differentiation; lung development; ossification; protein complex assembly; regulation of bone mineralization; response to calcium ion; response to glucocorticoid stimulus; response to hormone; response to mechanical stimulus; response to nutrient; response to organic substance

Research Articles on MGP

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Product Notes

The MGP mgp (Catalog #AAA2001703) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Matrix Gla Protein (MGP) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Matrix Gla Protein (MGP) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-5000 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the MGP mgp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Matrix Gla Protein (MGP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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