RLN2 recombinant protein

RLN2, 25-185aa, Human, His-tag, Baculovirus

Cat. Num. AAA206551

• Gene Names: RLN2; H2; RLXH2; H2-RLX; bA12D24.1.1; bA12D24.1.2
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: RLN2; 25-185aa; Human; His-tag; Baculovirus; Prorelaxin H2 isoform 1; bA12D24.1.1; bA12D24.1.2; H2; H2-RLX; RLXH2; RLN2 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPDSWMEEV IKLCGRELVR AQIAICGMST WSKRSLSQED APQTPRPVAE IVPSFINKDT ETINMMSEFV ANLPQELKLT LSEMQPALPQ LQQHVPVLKD SSLLFEEFKK LIRNRQSEAA DSSPSELKYL GLDTHSRKKR QLYSALANKC CHVGCTKRSL ARFCHHHHHH
• Sequence Length: 185

• Applicable Applications for RLN2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RLN2 recombinant protein

RLN2, as known as prorelaxin H2 isoform 1, belongs to the insulin gene superfamily. This family is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. It is 18 kDa in size and 185 aa in length. It contains a 24 aa signal sequence, a 3.3 kDa, 29 aa B domain, a 106 aa C (or connecting) domain, and a C-terminal, 2.7 kDa, 24 aa A domain. Recombinant human RLN2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for RLN2 recombinant protein

Signal Transduction; Hormones & Cytokines

References

Ng TM., et al, (2016) Cardiol Rev. 24:194-204.; Sherwood OD., et al, (2004) Endocr. Rev. 25:205-234.

NCBI and Uniprot Product Information

• NCBI GI #: 20143933
• NCBI GeneID: 6019
• NCBI Accession #: NP_604390
• NCBI GenBank Nucleotide #: NM_134441.2
• UniProt Accession #: P04090; Q99936; Q9UCX3; Q9UQJ2; A0AVM0
• Molecular Weight: 19.3kDa (170aa) 18-28kDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: prorelaxin H2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: relaxin 2
• NCBI Official Symbol: RLN2
• NCBI Official Synonym Symbols: H2; RLXH2; H2-RLX; bA12D24.1.1; bA12D24.1.2
• NCBI Protein Information: prorelaxin H2
• UniProt Protein Name: Prorelaxin H2
• Protein Family: Prorelaxin
• UniProt Gene Name: RLN2

NCBI Description

This gene encodes a member of the relaxin subfamily and insulin superfamily of peptide hormones. In humans there are three non-allelic relaxin genes. This gene encodes multiple protein isoforms, at least one of which undergoes proteolytic processing. This processing generates relaxin A and B chains that are linked by disulfide bonds to form the mature peptide hormone. This hormone plays a role in the male and female reproductive systems and was initially noted for its role in pregnancy. This protein also plays broader roles in the cardiovascular system, including in the regulation of blood pressure and control of heart rate, and data from animal models shows that this protein may have anti-fibrotic and cardioprotective effects. [provided by RefSeq, Jul 2016]

Uniprot Description

Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.