Goat anti-Human Mast Cell Protease I Polyclonal Antibody | anti-Mcpt1l1 antibody

Mast Cell Protease I, ID (Chymase, Alpha-chymase, CMA1, CYM, CYH)

Cat. Num. AAA6004422

• Gene Names: Mcpt1l1; Mcpt1; rMCP-1; rMCP-I
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Mast Cell Protease I; Polyclonal Antibody; ID (Chymase; Alpha-chymase; CMA1; CYM; CYH); Anti -Mast Cell Protease I; anti-Mcpt1l1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human CMA1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-Mcpt1l1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:32,000; Western Blot: 0.5-2ug/ml
• Immunogen: Synthetic peptide corresponding to C-QFRHPKYNTSTLHHD, from human CMA1, at the internal region of the protein (NP_001827.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(HEK293 lysate (10ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with = (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-Mcpt1l1 antibody

CMA1 is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this protein is a target for cardiovascular disease therapies.

Product Categories/Family for anti-Mcpt1l1 antibody

Antibodies; Abs to Cell Markers

NCBI and Uniprot Product Information

• NCBI GI #: 480306394
• NCBI GeneID: 100360872
• NCBI Accession #: NP_001264597.1
• NCBI GenBank Nucleotide #: NM_001277668.1
• UniProt Accession #: P09650
• Molecular Weight: 28,617 Da
• NCBI Official Full Name: mast cell protease 1 preproprotein
• NCBI Official Synonym Full Names: mast cell protease 1-like 1
• NCBI Official Symbol: Mcpt1l1
• NCBI Official Synonym Symbols: Mcpt1; rMCP-1; rMCP-I
• NCBI Protein Information: mast cell protease 1; chymase; CLIP protein; protease 1-like; mast cell protease I; chymotrypsin-like protease
• UniProt Protein Name: Mast cell protease 1
• UniProt Gene Name: Mcpt1
• UniProt Synonym Gene Names: rMCP-1; CLIP protein; rMCP-I
• UniProt Entry Name: MCPT1_RAT

Uniprot Description

Function: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. May participate in generating perivascular beta-protein which ultimately aggregates into beta-amyloid deposits. Ref.5

Catalytic activity: Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.

Subcellular location: Secreted

By similarity. Cytoplasmic granule

By similarity. Note: Mast cell granules

By similarity.

Sequence similarities: Belongs to the peptidase S1 family. Granzyme subfamily.Contains 1 peptidase S1 domain.