Western Blot (WB)
(Western Blot (WB) analysis of Maspin polyclonal antibody in extracts from HT-29 and NIH3T3 cells.)
Rabbit anti-Human, Mouse Maspin Polyclonal Antibody | anti-Maspin antibody
Maspin Polyclonal Antibody
Gene Names
SERPINB5; PI5; maspin
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Maspin; Polyclonal Antibody; Maspin Polyclonal Antibody; Serpin B5; Peptidase inhibitor 5; PI-5; SERPINB5; PI5; anti-Maspin antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Maspin polyclonal antibody detects endogenous levels of Maspin protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
375
Applicable Applications for anti-Maspin antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant full length Human Maspin.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Maspin polyclonal antibody in extracts from HT-29 and NIH3T3 cells.)
Western Blot (WB)
(Western Blot (WB) analysis of Maspin polyclonal antibody in extracts from HT-29 and NIH3T3 cells.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analysis of Maspin pAb in paraffin-embedded Human Colon tissue.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analysis of Maspin pAb in paraffin-embedded Human Colon tissue.)
Related Product Information for anti-Maspin antibody
Maspin is structurally a serine protease inhibitor (serpin) that was initially isolated from normal human mammary epithelial cells. Serpins are a family of proteins that inhibit chymotrypsin-like serine proteinases. Serpins control activated proteinases and several are involved in the regulation of cell death. Maspin is found in the extracellular matrix and at the plasma membrane. Maspin has been shown to act at the cell surface to block cell motility and inhibit invasion of breast and prostate cancer cells. Maspin is present in normal mammary epithelial cells but is absent in many tumor cell lines, yet no major structural alterations of the Maspin gene have been identified in tumor cells. Similarly, Maspin is expressed in normal prostate cells and downregulated or absent in prostate tumor cells..
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 42kDa
NCBI Official Full Name
serpin B5
NCBI Official Synonym Full Names
serpin family B member 5
NCBI Official Symbol
SERPINB5
NCBI Official Synonym Symbols
PI5; maspin
NCBI Protein Information
serpin B5
UniProt Protein Name
Serpin B5
UniProt Gene Name
SERPINB5
UniProt Synonym Gene Names
PI5; PI-5
UniProt Entry Name
SPB5_HUMAN
Research Articles on Maspin
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Product Notes
The Maspin serpinb5 (Catalog #AAA3007759) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Maspin Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Maspin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the Maspin serpinb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Maspin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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