Western Blot (WB)
(Western blot analysis of lysates from HeLa cells treated with EGF, using Paxillin (Phospho-Tyr118) Antibody. The lane on the right is blocked with the phospho peptide.)
Rabbit Paxillin Polyclonal Antibody | anti-PXN antibody
Paxillin (Phospho-Tyr118) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
Paxillin; Polyclonal Antibody; Paxillin (Phospho-Tyr118) Antibody; anti-PXN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Paxillin (Phospho-Tyr118) Antibody detects endogenous levels of Paxillin only when phosphorylated at Tyr118.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
424
Applicable Applications for anti-PXN antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:10000
ELISA: 1:10000
Target Modification
Phospho
Modification Sites
H:Y118; M:Y118; R:Y118
Immunogen
The antiserum was produced against synthesized peptide derived from human Paxillin around the phosphorylation site of Tyr118.
Immunogen Range
85-134
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Western Blot (WB)
(Western blot analysis of lysates from HeLa cells treated with EGF, using Paxillin (Phospho-Tyr118) Antibody. The lane on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from HeLa cells treated with EGF, using Paxillin (Phospho-Tyr118) Antibody. The lane on the right is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64 kDa
NCBI Official Full Name
paxillin isoform 2
NCBI Official Synonym Full Names
paxillin
NCBI Official Symbol
PXN
NCBI Protein Information
paxillin
UniProt Protein Name
Paxillin
Protein Family
UniProt Gene Name
PXN
NCBI Description
This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]
Uniprot Description
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Research Articles on PXN
Similar Products
Product Notes
The PXN pxn (Catalog #AAA9503136) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Paxillin (Phospho-Tyr118) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Paxillin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the PXN pxn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Paxillin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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