Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human STE20-like serine/threonine-protein kinase (SLK) Standard Curve.)
Human STE20-Like Serine/Threonine-Protein Kinase (SLK) ELISA Kit | SLK elisa kit
Human STE20-Like Serine/Threonine-Protein Kinase (SLK) ELISA Kit
Gene Names
SLK; LOSK; STK2; se20-9; bA16H23.1
Reactivity
Human
Synonyms
STE20-Like Serine/Threonine-Protein Kinase (SLK); Human STE20-Like Serine/Threonine-Protein Kinase (SLK) ELISA Kit; SLK; KIAA0204; LOSK; MGC133067; STK2; bA16H23.1; se20-9; CTCL tumor antigen se20-9; Long Ste20-like Kinase; SNF1 (sucrose nonfermenting; yeast; homolog)-like kinase; SNF1 sucrose nonfermenting like kinase; SLK elisa kit
Reactivity
Human
Specificity
Human STE20-like serine/threonine-protein kinase (SLK) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLK. No significant cross-reactivity or interference between Human SLK and analogues was observed.
Sequence Length
1235
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human STE20-like serine/threonine-protein kinase (SLK) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human STE20-like serine/threonine-protein kinase (SLK) Standard Curve.)
Related Product Information for SLK elisa kit
Description: This Human STE20-like serine/threonine-protein kinase (SLK) ELISA Kit employs a two-site sandwich ELISA to quantitate SLK in samples. An antibody specific for SLK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: FYN encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. Fyn is a tyrosine specific phospho-transferase that is a member of the Src family of tyrosine protein kinases.Fyn is primarily localized to the cytoplasmic leaflet of the plasma membrane, where it phosphorylates tyrosine residues on key targets involved in a variety of different signaling pathways. Tyrosine phosphorylation of target proteins by Fyn serves to either regulate target protein activity, and/or to generate a binding site on the target protein that recruits other signaling molecules.
Background: FYN encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. Fyn is a tyrosine specific phospho-transferase that is a member of the Src family of tyrosine protein kinases.Fyn is primarily localized to the cytoplasmic leaflet of the plasma membrane, where it phosphorylates tyrosine residues on key targets involved in a variety of different signaling pathways. Tyrosine phosphorylation of target proteins by Fyn serves to either regulate target protein activity, and/or to generate a binding site on the target protein that recruits other signaling molecules.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
STE20-like serine/threonine-protein kinase isoform 1
NCBI Official Synonym Full Names
STE20 like kinase
NCBI Official Symbol
SLK
NCBI Official Synonym Symbols
LOSK; STK2; se20-9; bA16H23.1
NCBI Protein Information
STE20-like serine/threonine-protein kinase
UniProt Protein Name
STE20-like serine/threonine-protein kinase
Protein Family
UniProt Gene Name
SLK
UniProt Synonym Gene Names
KIAA0204; STK2; STE20-like kinase; hSLK
UniProt Entry Name
SLK_HUMAN
Uniprot Description
SLK: a microtubule-associated protein kinase required for progression through G2. Co-localizes with the mitotic spindle in cells undergoing mitosis. Regulates actin reorganization during cell adhesion and spreading via a Rac1-mediated pathway. Signals via ASK1 and p38 to promote apoptosis. Activated in response to anoxia and ischemia-reperfusion injury in cell culture. It inhibits the protective aspects of the endoplasmic reticulum stress response, thus contributing to its proapoptotic effect. Two alternatively-spliced isoforms have been described.
Protein type: Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; STE group; STE20 family; SLK subfamily
Chromosomal Location of Human Ortholog: 10q24.33
Cellular Component: cytoplasm
Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein homodimerization activity; ATP binding; receptor signaling protein serine/threonine kinase activity
Biological Process: apoptosis; protein amino acid autophosphorylation
Research Articles on SLK
Similar Products
Product Notes
The Human SLK slk (Catalog #AAA9714244) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714244 ELISA Kit recognizes Human SLK. It is sometimes possible for the material contained within the vial of "STE20-Like Serine/Threonine-Protein Kinase (SLK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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