Rabbit anti-Human MAS1 Polyclonal Antibody | anti-MAS1 antibody

MAS1, CT (MAS1, MAS, Proto-oncogene Mas) (PE)

Cat. Num. AAA6319099

• Gene Names: MAS1; MAS; MGRA
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAS1; Polyclonal Antibody; CT (MAS1; MAS; Proto-oncogene Mas) (PE); Proto-oncogene Mas; anti-MAS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MAS1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa284-314 in the C-terminal region of human MAS1, UniProt Accession #NP_002368.1; P04201. Cellular Localization: Cell membrane; Multi-pass membrane protein
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MAS1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MAS1 antibody detected the MAS1 protein (arrow).)

Related Product Information for anti-MAS1 antibody

The structure of the MAS1 product indicates that it belongs to the class of receptors that are coupled to GTP-binding proteins and share a conserved structural motif, which is described as a '7-transmembrane segment' following the prediction that these hydrophobic segments form membrane-spanning alpha-helices. The MAS1 protein may be a receptor that, when activated, modulates a critical component in a growth-regulating pathway to bring about oncogenic effects.

Product Categories/Family for anti-MAS1 antibody

Antibodies; Proteins

References

Yang, J.K., et al. Diabetes Care 33(10):2271-2273(2010). Reis, A.B., et al. J. Mol. Histol. 41(1):75-80(2010). Trynka, G., et al. Gut 58(8):1078-1083(2009). Iwai, M., et al. Hypertens. Res. 32(7):533-536(2009). Vaz-Silva, J., et al. Reprod Sci 16(3):247-256(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4505105
• NCBI GeneID: 4142
• NCBI Accession #: NP_002368.1
• NCBI GenBank Nucleotide #: NM_002377.2
• UniProt Accession #: P04201; Q2TBC9; Q6FG47; E1P5B3
• Molecular Weight: 37,465 Da
• NCBI Official Full Name: proto-oncogene Mas
• NCBI Official Synonym Full Names: MAS1 proto-oncogene, G protein-coupled receptor
• NCBI Official Symbol: MAS1
• NCBI Official Synonym Symbols: MAS; MGRA
• NCBI Protein Information: proto-oncogene Mas; MAS1 oncogene; Mas-related G protein-coupled receptor A
• UniProt Protein Name: Proto-oncogene Mas
• Protein Family: Proto-oncogene
• UniProt Gene Name: MAS1
• UniProt Synonym Gene Names: MAS
• UniProt Entry Name: MAS_HUMAN

NCBI Description

This gene encodes a class I seven-transmembrane G-protein-coupled receptor. The encoded protein is a receptor for angiotensin-(1-7) and preferentially couples to the Gq protein, activating the phospholipase C signaling pathway. The encoded protein may play a role in multiple processes including hypotension, smooth muscle relaxation and cardioprotection by mediating the effects of angiotensin-(1-7). [provided by RefSeq, May 2012]

Uniprot Description

MAS1: Receptor for angiotensin 1-7. Acts specifically as a functional antagonist of AGTR1 (angiotensin-2 type 1 receptor), although it up-regulates AGTR1 receptor levels. Positive regulation of AGTR1 levels occurs through activation of the G-proteins GNA11 and GNAQ, and stimulation of the protein kinase C signaling cascade. The antagonist effect on AGTR1 function is probably due to AGTR1 being physically altered by MAS1. The MAS oncogene has a weak focus-inducing activity in transfected NIH 3T3 cells. Belongs to the G-protein coupled receptor 1 family.

Protein type: Oncoprotein; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 6q25.3-q26

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; angiotensin type II receptor activity; peptide hormone binding; peptide binding; angiotensin receptor activity

Biological Process: response to drug; anatomical structure morphogenesis; male gonad development; hippocampus development; activation of NF-kappaB-inducing kinase; cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of protein amino acid phosphorylation; regulation of inflammatory response; positive regulation of cell proliferation; spermatogenesis; response to activity; positive regulation of DNA replication