Rabbit anti-Rat Mas-related G-protein Coupled Receptor Member X1 Polyclonal Antibody | anti-MRGPRX1 antibody

Mas-related G-protein Coupled Receptor Member X1 (MAS-related GPR Member X1, MRGPRX1, MRGX1, Sensory Neuron-specific G-protein Coupled Receptor 3/4, SNSR3, SNSR4)

Cat. Num. AAA622435

• Gene Names: MRGPRX1; GPCR; MRGX1; SNSR4
• Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Mas-related G-protein Coupled Receptor Member X1; Polyclonal Antibody; Mas-related G-protein Coupled Receptor Member X1 (MAS-related GPR Member X1; MRGPRX1; MRGX1; Sensory Neuron-specific G-protein Coupled Receptor 3/4; SNSR3; SNSR4); Anti -Mas-related G-protein Coupled Receptor Member X1 (MAS-related GPR Member X1; anti-MRGPRX1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Mas-related G-protein coupled receptor member X1 at ~37kD.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 100ul sterile ddH2O.

• Applicable Applications for anti-MRGPRX1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.1-0.5ug/ml; Immunohistochemistry (formalin/paraffin): 0.5-1.0ug/ml; Heat mediated antigen retrieval is required.
• Immunogen: Synthetic peptide corresponding to aa14-28, TLNKTGHPSCRPILT, of rat Mas-related G-protein coupled receptor member X1, conjugated to BSA.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of MBS622435 to MRGPRX1 (0.75ug/ml): Lane 1: Lane marker, Lane 2: Rat heart tissue lysate, Lane 3: Rat skeletal muscle tissue lysate, Lane 4: MCG7 whole cell lysate Secondary HRP conjugated goat anti-rabbit IgG antibody (working dilution: 1:3000-1:10000).)

Related Product Information for anti-MRGPRX1 antibody

Mas-related G-protein coupled receptor member X1 (MRGPRX1) is an opiod receptor that is uniquely localized in a subset of small dorsal root and trigeminal sensory neurons. MRGPRX1 is thought to be involved in the regulation of sensory neurons.

Product Categories/Family for anti-MRGPRX1 antibody

Antibodies; Abs to G Protein Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 125660451
• NCBI GeneID: 259249
• NCBI Accession #: NP_671732.3
• NCBI GenBank Nucleotide #: NM_147199.3
• UniProt Accession #: Q96LB2; Q4V9L2; Q8TDD8; Q8TDD9
• Molecular Weight: 36,250 Da
• NCBI Official Full Name: mas-related G-protein coupled receptor member X1
• NCBI Official Synonym Full Names: MAS-related GPR, member X1
• NCBI Official Symbol: MRGPRX1
• NCBI Official Synonym Symbols: GPCR; MRGX1; SNSR4
• NCBI Protein Information: mas-related G-protein coupled receptor member X1; OTTHUMP00000222450; G protein-coupled receptor MRGX1; G protein-coupled receptor SNSR3; sensory neuron-specific G-protein coupled receptor 3/4
• UniProt Protein Name: Mas-related G-protein coupled receptor member X1
• Protein Family: Mas-related G-protein coupled receptor
• UniProt Gene Name: MRGPRX1
• UniProt Synonym Gene Names: MRGX1; SNSR3; SNSR4
• UniProt Entry Name: MRGX1_HUMAN

Uniprot Description

MRGPRX1: Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells. G(alpha)q proteins are involved in the calcium-signaling pathway. Activated by the antimalarial drug, chloroquine. May mediate chloroquine- induced itch, in an histamine-independent manner. Belongs to the G-protein coupled receptor 1 family. Mas subfamily.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11p15.1|11

Cellular Component: plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; acute-phase response