Western Blot (WB)
(The anti-MARK1 C-term Pab is used in Western blot to detect MARK1 in, from left to right, Hela, T47D, and mouse brain cell line/ tissue lysate.)
Rabbit anti-Human, mouse MARK1 Polyclonal Antibody | anti-MARK1 antibody
MARK1 Antibody (C-term)
Gene Names
MARK1; MARK; Par1c; Par-1c
Reactivity
Human, mouse
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MARK1; Polyclonal Antibody; MARK1 Antibody (C-term); Serine/threonine-protein kinase MARK1; MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c; Par-1c; Par1c; MARK1 HGNC:6896); anti-MARK1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MARK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 671-700 amino acids from the C-terminal region of human MARK1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
671-700
Sequence Length
795
Applicable Applications for anti-MARK1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(The anti-MARK1 C-term Pab is used in Western blot to detect MARK1 in, from left to right, Hela, T47D, and mouse brain cell line/ tissue lysate.)
Western Blot (WB)
(The anti-MARK1 C-term Pab is used in Western blot to detect MARK1 in, from left to right, Hela, T47D, and mouse brain cell line/ tissue lysate.)
Related Product Information for anti-MARK1 antibody
MARK is a family of kinases that is known for its involvement in establishing cell polarity and in phosphorylating tau protein during Alzheimer neurodegeneration. Expression of MARK causes the phosphorylation of MAPs at their KXGS motifs, thereby detaching MAPs from the microtubules and thus facilitating the transport of particles. This occurs without impairing the intrinsic activity of motors because the velocity during active movement remains unchanged. In primary retinal ganglion cells, transfection with tau leads to the inhibition of axonal transport of mitochondria, APP vesicles, and other cell components which leads to starvation of axons and vulnerability against stress. This transport inhibition can be rescued by phosphorylating tau with MARK
Product Categories/Family for anti-MARK1 antibody
References
Drewes, G., et al., Cell 89(2):297-308 (1997).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89003
NCBI Official Full Name
serine/threonine-protein kinase MARK1 isoform 2
NCBI Official Synonym Full Names
MAP/microtubule affinity-regulating kinase 1
NCBI Official Symbol
MARK1
NCBI Official Synonym Symbols
MARK; Par1c; Par-1c
NCBI Protein Information
serine/threonine-protein kinase MARK1
UniProt Protein Name
Serine/threonine-protein kinase MARK1
Protein Family
UniProt Gene Name
MARK1
UniProt Synonym Gene Names
Par-1c; Par1c
UniProt Entry Name
MARK1_HUMAN
Uniprot Description
MARK1: a member of the CAMKL family of protein kinases. The MARK group of kinases is apparently involved in the control of cell polarity, microtubule stability and cancer. MARK1 forms a tight association with Tau in neurofibrillary tangles in Alzheimer disease tissue.
Protein type: EC 2.7.11.26; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; EC 2.7.11.1; CAMK group; CAMKL family; MARK subfamily
Chromosomal Location of Human Ortholog: 1q41
Cellular Component: microtubule cytoskeleton; cytoskeleton; cytoplasm; plasma membrane
Molecular Function: protein serine/threonine kinase activity; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylserine binding; tau-protein kinase activity; magnesium ion binding; ATP binding
Biological Process: Wnt receptor signaling pathway; neuron migration; cytoskeleton organization and biogenesis; protein amino acid phosphorylation
Research Articles on MARK1
Similar Products
Product Notes
The MARK1 mark1 (Catalog #AAA9206665) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 671-700. The MARK1 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MARK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the MARK1 mark1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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