Rabbit anti-Human, Mouse MARK1 Polyclonal Antibody | anti-MARK1 antibody

MARK1, NT (MARK1, KIAA1477, MARK, Serine/threonine-protein kinase MARK1, MAP/microtubule affinity-regulating kinase 1, PAR1 homolog c) (Azide free) (HRP)

Cat. Num. AAA6319060

• Gene Names: MARK1; MARK; Par1c; Par-1c
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MARK1; Polyclonal Antibody; NT (MARK1; KIAA1477; MARK; Serine/threonine-protein kinase MARK1; MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c) (Azide free) (HRP); PAR1 homolog c; anti-MARK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MARK1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:200-500; Applications are based on unconjugated antibody.
• Immunogen: MARK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16~45 amino acids from the N-terminal region of human MARK1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-MARK1 N-term Pab is used in Western blot to detect MARK1 in P7 mouse whole brain lysate (60 ug). 1:250 dilution of anti-MARK1 pab was used. Data and protocol kindly provided by DR. Shengli Zhao, M.D., Ph.D. of Duke University Medical Center.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with MARK1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-MARK1 antibody

MARK is a family of kinases that is known for its involvement in establishing cell polarity and in phosphorylating tau protein during Alzheimer neurodegeneration. Expression of MARK causes the phosphorylation of MAPs at their KXGS motifs, thereby detaching MAPs from the microtubules and thus facilitating the transport of particles. This occurs without impairing the intrinsic activity of motors because the velocity during active movement remains unchanged. In primary retinal ganglion cells, transfection with tau leads to the inhibition of axonal transport of mitochondria, APP vesicles, and other cell components which leads to starvation of axons and vulnerability against stress. This transport inhibition can be rescued by phosphorylating tau with MARK

Product Categories/Family for anti-MARK1 antibody

Antibodies; Protein Kinases

References

Drewes, G., et al., Cell 89(2):297-308 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 121948901
• NCBI GeneID: 4139
• UniProt Accession #: Q2HIY1; Q9P0L2
• NCBI Official Synonym Full Names: microtubule affinity regulating kinase 1
• NCBI Official Symbol: MARK1
• NCBI Official Synonym Symbols: MARK; Par1c; Par-1c
• NCBI Protein Information: serine/threonine-protein kinase MARK1
• Protein Family: Serine/threonine-protein kinase