Western Blot (WB)
(Western blot analysis of MARCH9 using MCF7 whole cell lysates)
Rabbit anti-Human, Mouse MARCH9 Polyclonal Antibody | anti-MARCH9 antibody
MARCH9 Antibody
Gene Names
MARCH9; RNF179; MARCH-IX
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MARCH9; Polyclonal Antibody; MARCH9 Antibody; BC019560; E3 ubiquitin protein ligase MARCH9; FLJ36578; MARCH IX; Membrane associated RING CH protein IX; membrane associated ring finger (C3HC4) 9; Membrane associated RING finger protein 9; RING finger protein 179; RNF179; anti-MARCH9 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
MARCH9 antibody detects endogenous levels of total 42438
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
346
Applicable Applications for anti-MARCH9 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human MARCH9
Subcellular Location
Golgi Apparatus Membrane; Multipass Membrane Protein. Lysosome Membrane; Multipass Membrane Protein.
Tissue Specificity
Ubiquitously expressed.
Predicted Cross Reactivity
Pig
Similarity
Pig (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of MARCH9 using MCF7 whole cell lysates)
Western Blot (WB)
(Western blot analysis of MARCH9 using MCF7 whole cell lysates)
Related Product Information for anti-MARCH9 antibody
Function: E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I, CD4 and ICAM1, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Subunit Structure: Homodimer.
Similarity: The RING-CH-type zinc finger domain is required for E3 ligase activity.
Subunit Structure: Homodimer.
Similarity: The RING-CH-type zinc finger domain is required for E3 ligase activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 38 kDa
Predicted: 38 kDa
Predicted: 38 kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase MARCH9
NCBI Official Synonym Full Names
membrane associated ring-CH-type finger 9
NCBI Official Symbol
MARCH9
NCBI Official Synonym Symbols
RNF179; MARCH-IX
NCBI Protein Information
E3 ubiquitin-protein ligase MARCH9
UniProt Protein Name
E3 ubiquitin-protein ligase MARCH9
UniProt Gene Name
MARCH9
UniProt Synonym Gene Names
RNF179; MARCH-IX
NCBI Description
MARCH9 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH9 induces internalization of several membrane glycoproteins and directs them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Hoer et al., 2007 [PubMed 17174307]).[supplied by OMIM, Apr 2010]
Uniprot Description
E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I, CD4 and ICAM1, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Research Articles on MARCH9
Similar Products
Product Notes
The MARCH9 march9 (Catalog #AAA9609802) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MARCH9 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MARCH9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the MARCH9 march9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARCH9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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